Abstract Recently, rapid and effective extraction and purification methods have attracted considerable interest as alternative approaches to conventional methods. In this study, ultrasonic-microwave-assisted extraction (UMAE) was used to extract Gracilaria lemaneiformis polysaccharides, and the response surface methodology was used to determine the optimum extraction conditions. Subsequently, an aqueous two-phase system (ATPS) was applied to the purification of crude polysaccharides. The chemical structure and anti-tumor activities of three fractions of purified G. lemaneiformis polysaccharides were also investigated. We found that the optimal condition for UMAE was 50 W of ultrasonic power, 31.7 min, 87°C and at a solid-to-water ratio of 1.0:60.7 of extraction temperature. The extraction yield of polysaccharides reached 34.8% under optimum UMAE conditions. The crude polysaccharides were successfully partitioned in the intermediate phase with a recovery rate of 81.1% in an ethanol/ammonium sulfate system. After being fractionated by a DEAE Sephadex A-50 column, three polysaccharide fractions (GLP1, GLP2, and GLP3) were obtained, accounting for 14.3%, 65.2% and 20.6%, respectively. GLP1, GLP2 and GLP3 were mainly composed of galactose with molecular weights of 5.5 kDa, 85 kDa and 82 kDa, respectively. Those polysaccharides exhibited inhibition activity on MCF-7, HepG-2, and Hela cell proliferation in vitro , and GLP-3 exhibited the highest anti-tumor activities. These results indicated that UMAE and ATPS are rapid, efficient, and environmentally-friendly extraction and purification methods for marine algal polysaccharides. These G. lemaneiformis polysaccharides could potentially be used as anti-cancer adjuvants in conjunction with pharmaceutical therapies.
Last. Jianyong Wu(PolyU: Hong Kong Polytechnic University)H-Index: 69
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ABSTRACTIn recent years, a big trend has been the development of rapid, green, efficient, economical, and scalable approaches for the separation and purification of bioactive molecules from natural sources, which can be used in food, cosmetics, and medicine. As a new nonchromatographic bioseparation technology, three-phase partitioning (TPP) is attracting the attention of a growing number of scientists and engineers. Although a number of studies have been published in the last 40 years regarding...
Marine algae have attracted a great deal of interest as excellent sources of nutrients. Polysaccharides are the main components in marine algae, hence a great deal of attention has been directed at isolation and characterization of marine algae polysaccharides because of their numerous health benefits. In this review, extraction and purification approaches and chemico-physical properties of marine algae polysaccharides (MAPs) are summarized. The biological activities, which include immunomodulat...
Abstract The traditional technology replaced by ultrasonic or microwave assisted extraction has attracted considerable research interest. In order to maximize the yield of different degree polymerized oligosaccharides from lotus seeds, response surface methodology (RSM) was applied for optimization by ultrasound-microwave assisted extraction (UMAE) in this study. The results indicated that the optimal conditions for UMAE of lotus seed oligosaccharides were: extraction time 325.00 s, liquid-solid...
In this study, a simple aqueous two-phase system (ATPS) was employed for concurrent purification of oyster polysaccharides. The chemical structure and anti-tumor activities of purified oyster polysaccharides (OP-1) were also investigated. Under optimal ATPS conditions, oyster polysaccharides can be partitioned in the bottom phase with 67.02% extraction efficiency. The molecular weight of OP-1 was determined as 3480 Da. OP-1 is a (1→4)-α-d-glucosyl backbone and branching points located at O-3 of ...
#1Yinglang Ren(SCUT: South China University of Technology)H-Index: 2
#2Guiqing Zheng(SCUT: South China University of Technology)H-Index: 4
Last. Lin Zhou(SCUT: South China University of Technology)H-Index: 1
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Abstract Polysaccharides from Gracilaria lemaneiformis possess various beneficial bioactive functions, but the immunomodulatory activity remains incompletely known. In the present study, a new low molecular-weight sulfated polysaccharide named as GLP-2 was isolated from G. lemaneiformis. The chemical composition and structural characteristics of GLP-2 were determined by high-performance gel permeation chromatography (HPGPC), gas chromatography (GC), infrared (IR) spectrum, methylation and nuclea...
Last. Mingyong Xie(NCU: Nanchang University)H-Index: 71
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Abstract A water-soluble polysaccharide, named NCSP-50, was obtained from natural Cordyceps sinensis by hot water extraction and ethanol fractionation precipitation. It was eluted as a single symmetrical peak and had an average molecular weight of 9.76 × 10 5 Da. The structure was determined by monosaccharide composition, methylation analysis, 1D/2D NMR spectroscopy, and enzymatic hydrolysis and characterization of the oligosaccharides by MALDI-TOF mass spectrometry. The repeating unit of this p...
Gracilaria lemaneiformis (G. lemaneiformis) is rich in polysaccharides that have many functional activities. In this study, the thermostable α-amylase-assisted extraction and antioxidant activity of water-soluble polysaccharides from G. lemaneiformis were investigated. The extraction conditions were optimized as follows: time, 40 min; temperature, 95 °C; pH, 5 and enzyme amount, 6000 U/g, under which the yield of G. lemaneiformis polysaccharides (GPs) reached 49.15% dry base. The GPs were charac...
Last. Nianjun Xu(NBU: Ningbo University)H-Index: 5
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Substances with valuable antitumor properties have been identified in many marine algae, including an edible polysaccharide from the marine alga Gracilariopsis lemaneiformis (PGL). We previously reported transcriptome profiling data showing that PGL induced transcriptional alterations generate anti-lung cancer activity. To identify how PGL is detrimental to tumors, we purified PGL to characterize its chemical composition, molecular weight, and sugar and protein content and investigated its antit...
Last. Xiaolin Yan(STU: Shantou University)H-Index: 1
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Abstract The partitioning behaviors of several model proteins were investigated in aqueous micellar two-phase systems (AMTPS) composed of polyethylene glycol (PEG) with different molecular weight (4,000, 8000 and 20,000) and nonionic surfactant (TX-100), respectively. The influences of proteins molecular size or hydrophobicity, tie line length (TLL), compositions concentrations and PEG molecular weight on the partition coefficients of proteins were extensively studied. In order to evaluate the c...
Abstract Polygonum multiflorum is a popular Chinese herbal medicine with various pharmacological functions. In this study, the ultrasonic-assisted extraction condition, structural characterization and antitumor activity of a polysaccharide from roots of P. multiflorum were investigated. The ultrasonic-assisted extraction condition was optimized by single-factor experiments and response surface methodology. Results showed that the maximum extraction yield (5.49%) was obtained at ultrasonic power ...
#1Wanzi Yao(SCUT: South China University of Technology)
#2Xiaoyong Chen(SCUT: South China University of Technology)H-Index: 2
Last. Lijun You(SCUT: South China University of Technology)H-Index: 31
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Photoaging is a complex and multistage process triggered mainly by ultraviolet (UV) radiation due to exposure to sunlight. Photoaging induces DNA damage and oxidative stress that initiate an inflammatory response and an increase of matrix metalloproteinases (MMPs) expression, which results in cumulative changes in skin appearance, structure, and functions, and eventually causes skin carcinogenesis. Dietary polysaccharides from bio-resources have been utilized as functional ingredients in healthy...
Gracilariopsis lemaneiformis polysaccharides (GLP) were degraded using pectinase, glucoamylase, cellulase, xylanase, and β-dextranase into low-molecular-weight polysaccharides, namely, GPP, GGP, GCP, GXP, and GDP, respectively, and their antioxidant capacities were investigated. The degraded GLPs showed higher antioxidant activities than natural GLP, and GDP exhibited the highest antioxidant activity. After the optimization of degradation conditions through single-factor and orthogonal optimizat...
Abstract The sulfated polysaccharides extracted from Gracilaria chouae using critic acid extraction and water extraction, respectively, and their digestion and fermentation characteristics were compared in vitro and in vivo. The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73×103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa. During stimulated gastrointestinal digestion in vitro, WGCP and CGCP were lightly degraded. However, the glycemic index...
Marine algae contain abundant polysaccharides that support a range of health-promoting activities; however, the high molecular weight, high viscosity, and low solubility of marine algae polysaccharides (MAPs) limit their application in food, agriculture and medicine. Thus, as the degradation products of MAPs, marine algae oligosaccharides (MAOs) have drawn increasing attention. Most MAOs are non-digestible by digestive enzyme in the human gastrointestinal tract, but are fermented by bacteria in ...
Last. Xuguang Qiao(SDAU: Shandong Agricultural University)H-Index: 17
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Abstract Background The discovery of nutritious dietary supplements and side effect-free therapeutics are a priority in the current scenario of increasing cancer cases. Natural anticancer polysaccharides have shown immense potential. As a class of biomacromolecules produced by a variety of species including plants, animals, fungi, bacteria and algae, polysaccharides have drawn considerable interest over the recent years as they possess anticancer activities with fewer side effects. Scope and app...
In this study, Spirulina platensis (S.p.) polysaccharide (PSP) was obtained by ultrasonic-microwave-assisted extraction (UMAE) and purified by an aqueous two-phase system (ATPS). Two different methods were applied to purified Spirulina platensis (S.p.) polysaccharide (PSP), respectively, due to PSP as a complex multi-component system. Three polysaccharide fractions (PSP-1, PSP-2, and PSP-3) with different acidic groups were obtained after PSP was fractionated by the diethyl aminoethyl (DEAE)-52 ...
Type-II diabetes mellitus (T2DM) has become one of the most prevalent diseases on Earth and some treatments have been developed to manage it. One intestinal enzyme α-amylase can break down starch to glucose. Inhibiting its activity will control blood glucose and provide an essential approach for the management of T2DM. Alpha-amylase inhibitor (α-AI) specifically inhibits the activity of α-amylase, and reduces the blood glucose level efficiently. To develop a novel α-AI, the red seaweed laver (Po...
Abstract Herein, for the first time, the novel polysaccharides isolated from marine red algae Amphiroa rigida (ARPS) and enhanced its antioxidant, antibacterial, cytotoxicity, and apoptotic potential. An ultra-sonication disruption (USD) technology was employed to extract the ARPS and the extraction conditions were optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). The maximum ARPS yield (28.6%) was achieved with the solid-liquid ratio (1:70 mL), ultra-sonica...
To address the structure-activity relationship of Chlamys farreri polysaccharides on their immunostimulatory efficacy, two polysaccharides (CFP-1 and CFP-2) were extracted from Chlamys farreri by hot water extraction, and separated through column chromatography. The isolated CFPs were chemically analyzed to clarify their physicochemical characteristics and cultured with murine macrophage RAW264.7 cells, in order to evaluate their immunostimulatory efficacy. Despite the fact that both CFP-1 and C...