Bsa Adsorption on Porous Scaffolds Prepared from BioPegylated Poly(3-Hydroxybutyrate)

Published on Feb 8, 2018in Applied Biochemistry and Microbiology1.022
· DOI :10.1134/S0003683818040038
Anton P. Bonartsev13
Estimated H-index: 13
(MSU: Moscow State University),
V. V. Voinova11
Estimated H-index: 11
(MSU: Moscow State University)
+ 8 AuthorsGarina A. Bonartseva14
Estimated H-index: 14
(RAS: Russian Academy of Sciences)
Porous scaffolds for tissue engineering have been prepared from poly(3-hydroxybutyrate) (PHB) and a copolymer of poly(3-hydroxybutyrate) and polyethylene glycol (PHB-PEG) produced by bioPEGylation. The morphology of the scaffolds and their capacity for adsorption of the model protein bovine serum albumin (BSA) have been studied. Scaffolds produced from bioPEGylated PHB adsorbed more BSA, whereas the share of protein irreversibly adsorbed on these scaffolds was significantly lower (33%) than in the case of PHB homopolymer-based scaffolds (47%). The effect of protein adsorption on scaffold biocompatibility in vitro was tested in an experiment that involved the cultivation of fibroblasts (line COS-1) on the scaffolds. PHB-PEG scaffolds had a higher capacity for supporting cell growth than PHB-based scaffolds. Thus, the bioPEGylated PHB-based polymer scaffolds developed in the present study have considerable potential for use in soft tissue engineering.
📖 Papers frequently viewed together
13 Citations
19 Citations
#1Janice Lim (NUS: National University of Singapore)H-Index: 1
#2Mingliang You (NUS: National University of Singapore)H-Index: 6
Last. Zibiao Li (Agency for Science, Technology and Research)H-Index: 50
view all 4 authors...
Abstract Polyhydroxyalkanoates (PHAs) are a class of biodegradable polymers derived from microorganisms. On top of their biodegradability and biocompatibility, different PHA types can contribute to varying mechanical and chemical properties. This has led to increasing attention to the use of PHAs in numerous biomedical applications over the past few decades. Bone tissue engineering refers to the regeneration of new bone through providing mechanical support while inducing cell growth on the PHA s...
74 CitationsSource
#1E. R. Pavlova (MIPT: Moscow Institute of Physics and Technology)H-Index: 5
#2Dmitry V. Bagrov (MSU: Moscow State University)H-Index: 11
Last. Dmitry V. KlinovH-Index: 18
view all 11 authors...
7 CitationsSource
#1Antoine VenaultH-Index: 21
#2Andre SubarjaH-Index: 1
Last. Yung ChangH-Index: 55
view all 3 authors...
We present a method for surface modification by thermal-evaporation self-assembling of poly(3-hydroxybutyrate) (PHB) fibrous membranes with a copolymer of hydrophobic octadecyl acrylate repeat units and hydrophilic zwitterionic 4-vinylpyridine blocks, zP(4VP-r-ODA), in view of controlling biofoulant–fiber interactions. PHB is of interest as a material for bioscaffolding, but its disadvantage is its hydrophobicity, which leads to unwanted interactions with proteins, blood cells, or bacteria. Surf...
6 CitationsSource
#1Anton P. Bonartsev (Nizhny Novgorod State Medical Academy)H-Index: 13
#2I. I. Zharkova (MSU: Moscow State University)H-Index: 8
Last. Garina A. Bonartseva (RAS: Russian Academy of Sciences)H-Index: 14
view all 13 authors...
ABSTRACTA precursor feeding strategy for effective biopolymer producer strain Azotobacter chroococcum 7B was used to synthesize various poly(3-hydroxybutyrate) (PHB) copolymers. We performed experiments on biosynthesis of PHB copolymers by A. chroococcum 7B using various precursors: sucrose as the primary carbon source, various carboxylic acids and ethylene glycol (EG) derivatives [diethylene glycol (DEG), triethylene glycol (TEG), poly(ethylene glycol) (PEG) 300, PEG 400, PEG 1000] as additiona...
12 CitationsSource
#1Alexandre Monnier (University of Lyon)H-Index: 2
#2Charlotte Rombouts (AMU: Aix-Marseille University)H-Index: 8
Last. Nida Sheibat-Othman (University of Lyon)H-Index: 18
view all 6 authors...
The in vivo effectiveness of biomolecules may be limited by their rapid diffusion in the body and short half-life time. Encapsulation of these biomolecules allows protecting them against degradation and ensuring a controlled release over time. In this work, the production of polyhydroxybutyrate-co-hydroxyvalerate/polyethylene glycol-based microspheres loaded with heparin by double emulsion-solvent evaporation is investigated. Significant improvements are achieved after blending PHB-HV microspher...
13 CitationsSource
14 Citations
#1Rosetta N. Reusch (MSU: Michigan State University)H-Index: 26
The hypothesis is that poly-(R)-3-hydroxybutyrates (PHB), linear polymers of the ketone body, R-3-hydroxybutyrate (R-3HB), are atherogenic components of lipoprotein Lp(a). PHB are universal constituents of biological cells and are thus components of all foods. Medium chain-length PHB ( 70%) are absorbed by albumin, which transports them to the liver for disposal. When the amount of PHB in the diet exceed the capacity of albumin to safely remove them from the circulation, the excess PHB remain in...
4 CitationsSource
#1Jiezhao ZhanH-Index: 6
#2Lin WangH-Index: 20
Last. Yingjun WangH-Index: 42
view all 6 authors...
In this article, we prepared hyaluronic acid/poly(amidoamine) dendrimer (HA/PAMAM) multilayers on a poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-4HB)] substrate by a layer-by-layer self-assembly method for antimicrobial biomaterials. The results of ζ potential and quartz crystal microbalance with dissipation (QCM-D) showed that HA/PAMAM multilayers could be formed on the substrate layer by layer. We used QCM-D to show that both the HA outer layer and the PAMAM outer layer exhibited good p...
23 CitationsSource
#1Maraolina Domínguez-Díaz (UNAM: National Autonomous University of Mexico)H-Index: 6
#2Angélica Meneses-Acosta (Universidad Autónoma del Estado de Morelos)H-Index: 10
Last. Guadalupe Espín (UNAM: National Autonomous University of Mexico)H-Index: 29
view all 6 authors...
Abstract The thermo-mechanical properties, microstructure, and biocompatibility of a molecular weight series of poly-β-hydroxybutyrate (PHB) produced by Azotobacter vinelandii wild-type strain (OP) and mutant derivatives strains OPN, SOP1 and OPNA were determined. A broad molecular weight range was investigated, spanning from 160 kDa to 2030 kDa. Thermal properties were determined by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The results showed that the melting...
38 CitationsSource
#1Qiang Peng (Sichuan University)H-Index: 26
#2Xue-Qin WeiH-Index: 16
Last. Yunfeng LinH-Index: 50
view all 9 authors...
Aims: The long-term efficacy of nanoparticles is limited by their rapid metabolism in tissues. In this work, we aim to enhance nanoparticle biostability by preforming a bovine serum albumin (BSA) corona. Materials & methods: A BSA corona was formed by incubating poly-3-hydroxybutyrate-co-3-hydroxyhexanoate nanoparticles with BSA solution and confirmed by SDS-PAGE and x-ray photoelectron spectroscopy. The impacts of the BSA corona on the drug release, biostability and biodistribution of nanoparti...
39 CitationsSource
Cited By1
#1R. Abhinandan (SRM University)H-Index: 2
#2S. Pranav Adithya (SRM University)H-Index: 1
Last. Nagarajan Selvamurugan (SRM University)H-Index: 59
view all 5 authors...
Abstract Boride, which belongs to the distinct category of ceramic materials, has attracted significant attention in tissue engineering applications. Magnesium diboride (MgB2) consists of a plane of magnesium atoms sandwiched between the layers of boron. Even though MgB2 showed its role in various applications, its effect on osteogenesis has not yet been investigated. In this study, we synthesized MgB2 nanosheets (MgB2NS), a new class of 2D-nanoscale structures, by the ultrasonication exfoliatio...