Gene

Inosine

Biology

ADAR

Computer graphics (images)

Endocrinology

Operating system

Biochemistry

Computer science

Snap

Computational biology

Genetics

RNA editing

Adenosine

Endogeny

Nature Methods

Molecular tools that target RNA at specific sites allow recoding of RNA information and processing. SNAP-tagged deaminases guided by a chemically stabilized guide RNA can edit targeted adenosine to inosine in several endogenous transcripts simultaneously, with high efficiency (up to 90%), high potency, sufficient editing duration, and high precision. We used adenosine deaminases acting on RNA (ADARs) fused to SNAP-tag for the efficient and concurrent editing of two disease-relevant signaling transcripts, KRAS and STAT1. We also demonstrate improved performance compared with that of the recently described Cas13b-ADAR.

Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs