Rapid characterization of the chemical constituents of Sijunzi decoction by UHPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry.
Abstract Sijunzi decoction, a renowned Chinese prescription has long been utilized to treat gastrointestinal problems. In the context of this research work, the use of Ultra high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry was made to separate and characterize the components of Sijunzi decoction. The performance of Liquid chromatography was carried out on a C8 column (150 mm × 2.1 mm, 1.8 μm); moreover, the mobile phase were consisted of 0.2% formic acid (A) and acetonitrile (B). In accordance with the findings, characterization of 120 chemical compounds was performed by liquid chromatography with mass spectrometry. The key constituents among them included ginsenosides (in Radix Ginseng), 16 triterpene carboxylic acids (in Poria), sesquiterpenes (in Rhizoma Atractylodis Macrocephalae), triterpenesaponins (in Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle) as well as flavonoids (in Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle) in Sijunzi decoction. This research developed the bases for prospective research associated with Sijunzi decoction, together with being expected to be useful to rapidly extract and characterize the constituents in other Traditional Chinese herbal formulations.
#1Yangyang Xu(Nanjing University of Chinese Medicine)H-Index: 4
#2Hao Cai(Nanjing University of Chinese Medicine)H-Index: 12
Last. Lin Shen(Nanjing University of Chinese Medicine)H-Index: 3
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Abstract Baizhu Shaoyao San (BSS) is a famous traditional Chinese medicinal formula widely used for the treatment of painful diarrhea, intestinal inflammation, and diarrhea-predominant irritable bowel syndrome. According to clinical medication, three medicinal herbs (Atractylodis Macrocephalae Rhizoma, Paeoniae Radix Alba, and Citri Reticulatae Pericarpium) included in BSS must be processed using some specific methods of stir-frying. On the basis of the classical theories of traditional Chinese ...
Last. Zhiguo Yu(SPU: Shenyang Pharmaceutical University)H-Index: 16
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Abstract Gegenqinlian decoction (GQLD) as a traditional Chinese medical (TCM) prescription is made from four Chinese herbs and has been used for centuries to treat pyrexia and diarrhea. In this work, an ultra-high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) method was established to profile the metabolic fate of GQLD in rat plasma, urine, bile and feces. The analysis was performed on a Waters Acquity UPLC HSS T3 col...
Last. Xiu-Wei Yang(PKU: Peking University)H-Index: 24
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This study was conducted to determine the variations of ginsenosides in Ginseng Radix et Rhizoma when using different preparation solvents and explore the major factors for changes. With an established ultra-fast liquid chromatography coupled with tandem mass spectrometry method which could quantify 52 ginsenosides, the extraction differences were characterized and compared using different solvents (water, 70% aqueous ethanol, and ethanol). Subsequently, a series of aqueous solutions with differ...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 6
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Abstract Ethnopharmacological relevance Sijunzi decoction (SJZD) is a well known traditional Chinese prescription used for the treatment of gastrointestinal disorders and immunity enhancement. It has been found to indeed improve life quality of chemotherapy patients and extensive used in clinical conbined with chemotherapeutics for the treatment of cancer. Aim of the study The aim of this study was to investigate the preventive effect of the immunotoxicity of SJZD on mitomycin C (MMC) and the me...
The effective production and usage of ginsenosides, given their distinct pharmacological effects, are receiving increasing amounts of attention. As the ginsenosides content differs in different parts of Panax ginseng, we wanted to assess and compare the ginsenosides content in the ginseng roots, leave, stems, and berries. To extract the ginsenosides, 70% (v/v) methanol was used. The optimal ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) method ...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 5
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Cortex Fraxini is an important traditional Chinese medicine. In this work, a rapid and reliable homogenate extraction method was applied for the fast extraction for Cortex Fraxini, and the method was optimized by response surface methodology. Ultra high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry and gas chromatography with mass spectrometry were established for the separation and characterization of the constituents of Cortex Fraxi...
#1Byong kyu Shin(SNU: Seoul National University)H-Index: 4
#2Sung Won Kwon(SNU: Seoul National University)H-Index: 31
Last. Jeong Hill Park(SNU: Seoul National University)H-Index: 52
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Ginseng, a perennial plant belonging to the genus Panax of the Araliaceae family, is well known for its medicinal properties that help alleviate pathological symptoms, promote health, and prevent potential diseases. Among the active ingredients of ginseng are saponins, most of which are glycosides of triterpenoid aglycones. So far, numerous saponins have been reported as components of Panax ginseng, also known as Korean ginseng. Herein, we summarize available information about 112 saponins relat...
Last. Xiao-Qing Chen(CSU: Central South University)H-Index: 1
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Abstract Glycyrrhiza uralensis , an important Chinese medicine, has a long history of use in China. In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography. Physicochemical properties and antioxidant activities of the three purified polysaccharides were investigated. The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and ...
Last. Tianzhu Jia(LNUTCM: Liaoning University of Traditional Chinese Medicine)H-Index: 2
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Abstract Bai-Zhu, the dried rhizome of Atractylodes macrocephala Koidz (AMK), is widely used as a tonic herbal in eastern Asia. It is commonly used as prepared slices in clinic by stir-frying with wheat bran (processed AMK). In the theories of traditional Chinese medicine (TCM), Bai-Zhu possesses significantly different therapeutic effects before and after processing. However, the molecular mechanics of this processing is still unknown. In this paper, the strategy of metabolomics was employed to...
Thirteen new oleanane-type triterpenoid saponins, uralsaponins M–Y (1–13), and 15 known analogues (14–28) were isolated from the roots of Glycyrrhiza uralensis Fisch. The structures of 1–13 were identified on the basis of extensive NMR and MS data analyses. The sugar residues were identified by gas chromatography and ion chromatography coupled with pulsed amperometric detection after hydrolysis. Saponins containing a galacturonic acid (1–3) or xylose (5) residue are reported from Glycyrrhiza spe...
Shensu IV is a Chinese prescription well-known for its function in treating chronic kidney diseases. However, the potential mechanisms underlying how Shensu Ⅳ exerts its effects remain unclear. In this study, we investigated the effects of Shensu IV on glomerular podocyte injury in nephrotic rats and puromycin-induced injury in cultured podocytes, and assessed the associated molecular mechanisms. LC-MS results showed that the main components of Shensu IV were L-Carnitine, P-lysoPC (LPC) 16:0, Co...
#1Hai-Ming An(CPU: China Pharmaceutical University)H-Index: 2
#2Meng-Ning Li(CPU: China Pharmaceutical University)H-Index: 7
Last. Wen Gao(CPU: China Pharmaceutical University)H-Index: 13
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Abstract A rapid ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UHPLC-QqQ MS/MS) approach with high sensitivity and selectivity was developed for the quantification of twenty compounds, including 9 saponins, 8 flavonoids, 2 oligosaccharide esters and 1 phenolic acid, in rat plasma and brain, which was administrated intragastrically with Jia-Wei-Qi-Fu-Yin (JWQFY), Mass spectrometric detection was operated under multiple reaction monitoring (M...
Last. Xiaobo Li(SJTU: Shanghai Jiao Tong University)H-Index: 17
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Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases. While there are reports on pharmaceutical substances of SJZD focusing on its polysaccharides, the composition of non-polysaccharides (NPSs) has not yet been holistically clarified. In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for ...
Last. Zhihong Yao(JNU: Jinan University)H-Index: 16
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Wendan decoction, a classical traditional Chinese medicine formula consisting of six herbal medicines, has been widely used in clinical treatments for thousands of years due to the expectorant effects. However, the chemical basis of Wendan decoction remains unclear, which hinders the elucidation of the scientific connotation and mechanism of its effective components. In this study, an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry met...
Abstract In this work, an analytical model based on near infrared spectroscopy is proposed to predict the main active ingredients of Poria cocos (PC) during the extraction process, acidic polysaccharides. In order to improve the prediction quality, various pretreatment methods and variable selection methods were carefully compared and the partial least squares method was used to relate the predicted values to the reference ones. As a result, a robust and optimal model was obtained, where the coe...
Abstract Modern chromatography - mass spectrometer (MS) technology is an essential weapon in the exploration of traditional Chinese medicines (TCMs) which is based on the ‘effectiveness-material basis-quality markers (Q-markers)’. Nevertheless, the hardware bottleneck and irregular operation will limit the accuracy and comprehensiveness of test results. Chemometrics was thereby used to solve the existing problems: 1) The method of ‘design-modeling-optimization’ can be adopted to solve the multi-...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 6
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Shaoyao Gancao decoction (SGD), a well-known Chinese herbal formula, has been used to treat liver injury for a long time. In this study, chemical profiles of SGD were identified using ultra high-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS/MS). Liquid chromatography was performed on a C18 column (150 mm × 2.1 mm, 1.8 μm); the mobile phase comprised 0.1% formic acid (A) and acetonitrile (B). We then characterized 73 c...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 6
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Sanhua decoction, a famous Chinese herbal formula has been widely used for the treatment of stroke. In our study, a rapid, swift and straightforward analytical method with the help of UHPLC-FT-ICR-MS/MS was successfully developed for the first time to separate and identify the chemical constituents of Sanhua decoction. Chromatography was performed on a Universal XB C18 column (150 mm × 2.1 mm, 1.8 μm) using a mobile phase containing 0.1% formic acid–water (A) and acetonitrile (B). A total of 137...
Last. Zhiguo Yu(SPU: Shenyang Pharmaceutical University)H-Index: 16
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Abstract Gansuibanxia decoction (GSBXD) is one of the most famous traditional Chinese medicine (TCM). It is a herbal formula used for treating hydrops, such as cancerous ascites, pleural effusion, pericardial effusion, etc. However, the chemical constituents of GSBXD were still unclear. In this study, an UHPLC-FT-ICR-MS method was established and applied to the separation and characterization of the chemical constituents of GSBXD. A total of 62 components were chemically defined or tentatively i...
#1Qinghua Yan(Xinxiang Medical University)H-Index: 1
#2Huili Mao(Xinxiang Medical University)H-Index: 1
Last. Jianyi Guan(Xinxiang Medical University)H-Index: 1
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Abstract Sijunzi decoction (SJZD) is a traditional Chinese medicine prescription which is used to treat spleen deficiency syndrome. Its mechanism of action was studied in a rat model of reserpine-induced spleen deficiency syndrome with metabolomics assays performed in normal control, model and Sijunzi decoction treated rats by liquid chromatography quadruple-time-of-flight mass spectrometry (LC-Q/TOF-MS). Enzyme assays and histopathology, with multivariate statistical analysis, showed that Sijun...