Genome

Typing

Human genome

Gene

Antigen

Human leukocyte antigen

Biology

Genetics

Allele

Human genetics

Computational biology

Genome biology

Accurate typing of human leukocyte antigen (HLA) is important because HLA genes play important roles in immune responses and disease genesis. Previously available computational methods are database-matching approaches and their outputs are inherently limited by the completeness of already known types, making them unsuitable for discovery of novel alleles. We have developed a graph-guided assembly technique for classical HLA genes, which can construct allele sequences given high-coverage whole-genome sequencing data. Our method delivers highly accurate HLA typing, comparable to the current state-of-the-art methods. Using various data, we also demonstrate that our method can type novel alleles.

Kourami: graph-guided assembly for novel human leukocyte antigen allele discovery