Mass spectrometry

Chemistry

Ionization

Chromatography

Physics

Hydrogen–deuterium exchange

Molecule

Quantum mechanics

Organic chemistry

Deuterium

Hydrogen

Analytical Chemistry (journal)

Chemical ionization

Mass Spectrometry Reviews

The isotopic exchange approach is in use since the first observation of such reactions in 1933 by Lewis. This approach allows the investigation of the pathways of chemical and biochemical reactions, determination of structure, composition, and conformation of molecules. Mass spectrometry has now become one of the most important analytical tools for the monitoring of the isotopic exchange reactions. Investigation of conformational dynamics of proteins, quantitative measurements, obtaining chemical, and structural information about individual compounds of the complex natural mixtures are mainly based on the use of isotope exchange in combination with high resolution mass spectrometry. The most important reaction is the Hydrogen/Deuterium exchange, which is mainly performed in the solution. Recently we have developed the approach allowing performing of the Hydrogen/Deuterium reaction on‐line directly in the ionization source under atmospheric pressure. Such approach simplifies the sample preparation and can acce...

Hydrogen/deuterium exchange in mass spectrometry