Nanotechnology

Enzyme

Combinatorial chemistry

High-throughput screening

Microfluidics

Biology

Directed evolution

Gene

Mutant

Enantioselective synthesis

Catalysis

Chemistry

Materials science

Esterase

Computer science

Computational biology

Directed Molecular Evolution

Biochemistry

Nature Communications

Directed evolution has long been a key strategy to generate enzymes with desired properties like high selectivity, but experimental barriers and analytical costs of screening enormous mutant libraries have limited such efforts. Here, we describe an ultrahigh-throughput dual-channel microfluidic droplet screening system that can be used to screen up to ~10

Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform