Transcriptome

Immunoprecipitation

Biology

Gene

Gene expression

RNA-binding protein

Computational biology

Genetics

Molecular Cell

UV crosslinking and immunoprecipitation (CLIP) and related methods identify transcriptome-wide protein-RNA binding or RNA modification sites in vivo. Lee and Ule highlight the core steps of CLIP methods and discuss how the experimental variations in each published protocol affect the sensitivity, resolution, and specificity of the assigned crosslink sites.

Advances in CLIP Technologies for Studies of Protein-RNA Interactions