Qualitatively and quantitatively comparing secondary metabolites in three medicinal parts derived from Poria cocos (Schw.) Wolf using UHPLC-QTOF-MS/MS-based chemical profiling

Published on Feb 20, 2018in Journal of Pharmaceutical and Biomedical Analysis3.209
· DOI :10.1016/J.JPBA.2017.11.066
Lixia Zhu6
Estimated H-index: 6
(CDUTCM: Chengdu University of Traditional Chinese Medicine),
Jun Xu16
Estimated H-index: 16
(Hong Kong Baptist University)
+ 5 AuthorsZhongzhen Zhao50
Estimated H-index: 50
(Hong Kong Baptist University)
Abstract Poria cocos (Schw.) Wolf, is a fungus that grows on dead pine roots, producing a solid white sclerotium (fruiting body). Three parts of the sclerotium are commonly used in traditional Chinese medicine, namely, the epidermis, known as Poria Cutis or fulingpi in Chinese; the middle part, known as Poria or baifuling ; and the sclerotium with some host attached, known as fushen . For fushen , the hostwood is the inner part, known as fushenmu . The epidermis, middle part and middle-plus-inner parts have long been used for different syndromes or diseases, but the differences in their chemistry have not been established. In this study, ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS) was used to explore the differences of secondary metabolites in these three botanical parts of Poria cocos . In 13 batches of samples, a total of 147 chromatographic peaks were unequivocally or tentatively identified. Of these, 15 peaks were common to all three parts (the epidermis, middle, and inner part), while the rest were found in only one or two of the parts. Meanwhile, dissimilarities among the chemical compositions of three botanical parts were further investigated by principal component analysis (PCA), and supervised orthogonal partial least squares-discriminate analysis (OPLS-DA). Eight major bioactive triterpene acids that played important roles in differentiating the epidermis, middle and inner part were simultaneously quantified using the extracting ion mode of UHPLC-QTOF-MS/MS. The quantitative results showed that the analytes accumulated mostly in the epidermis and inner part, with the least accumulation in the middle part. This systematic information on the differences in secondary metabolites in the three botanical regions of Poria cocos provides a factual chemical basis not only for discrimination in therapeutic practice but also for quality evaluation of Poria cocos .
📖 Papers frequently viewed together
5 Authors (Bing Xia, ..., Hong-Xi Xu)
23 Citations
84 Citations
63 Citations
#1Lingfang Wu (Beijing University of Chinese Medicine)H-Index: 6
#2Kun-Feng Wang (Beijing University of Chinese Medicine)H-Index: 1
Last. Lanzhen Zhang (Beijing University of Chinese Medicine)H-Index: 8
view all 9 authors...
The aim of the present study was to establish a new method based on Similarity Analysis (SA), Cluster Analysis (CA) and Principal Component Analysis (PCA) to determine the quality of different samples of Poria cocos (Schw.) Wolf obtained from Yunnan, Hubei, Guizhou, Fujian, Henan, Guangxi, Anhui and Sichuan in China. For this purpose 15 samples from the different habitats were analyzed by HPLC-PAD and HPLC-MSn. Twenty-three compounds were detected by HPLC-MSn, of which twenty compounds were tent...
20 CitationsSource
#1Hongjing Dong (Peking Union Medical College)H-Index: 2
#1Hongjing DongH-Index: 5
Last. Bin YangH-Index: 4
view all 8 authors...
Triterpene acids were extracted from the epidermis of Poria cocos (Schw.) Wolf. These acids were found to inhibit the growth of lung cancer cells in vitro and in vivo. An efficient method for the preparative separation of antitumor triterpene acids was established that involves the combination of pH-zone-refining counter-current chromatography and conventional high-speed counter-current chromatography. We used pH-zone-refining counter-current chromatography to concentrate the triterpene acids us...
14 CitationsSource
#1Weihao WangH-Index: 4
#2Hongjing Dong (Peking Union Medical College)H-Index: 2
Last. Zhi-Min Wang (Peking Union Medical College)H-Index: 12
view all 8 authors...
Poria cocos (Schw.) Wolf is widely used as a traditional Chinese medicine, and approximately 10% of traditional Chinese medicinal preparations contain this material, according to the Chinese Pharmacopoeia (2010 edition). Although the epidermis (fulingpi in Chinese, or FLP) and the inner parts (baifuling in Chinese, or BFL) of P. cocos had different therapeutic applications in history, studies pertaining to a comparative analysis of their chemical constituents have been scarce. UHPLC–DAD–FT/MSn h...
32 CitationsSource
#1Amira S. El Senousy (Cairo University)H-Index: 4
#2Mohamed A. Farag (Cairo University)H-Index: 36
Last. Ludger A. Wessjohann (Leibniz Association)H-Index: 62
view all 4 authors...
Abstract The metabolomic differences in phenolics from leaves derived from 3 artichoke cultivars ( Cynara scolymus ): American Green Globe, French Hyrious and Egyptian Baladi, collected at different developmental stages, were assessed using UHPLC–MS coupled to chemometrics. Ontogenic changes were considered as leaves were collected at four different time intervals and positions (top and basal) during artichoke development. Unsupervised principal component analysis (PCA) and supervised orthogonal...
24 CitationsSource
#1Hsiu Chuan Lee (TMU: Taipei Medical University)H-Index: 6
#2Wen Yi Cheng (TMU: Taipei Medical University)H-Index: 3
Last. Shih Yi Huang (TMU: Taipei Medical University)H-Index: 27
view all 5 authors...
Poria cocos (Fu Ling) and Dioscorea opposita (Chinese Yam) were suggested to have potential benefits in blood sugar control. However, little is known about the underlying mechanisms. In this study, we investigated the anti-inflammatory and hypoglycemic effects of Poria cocos and Dioscorea opposita extracts in prediabetic rats. Fifty streptozotocin-injected rats with a mimic prediabetic status were gavaged with a single dose of either D. opposita (0.35 g kg−1 per day) or P. cocos (0.14 g kg−1 per...
13 CitationsSource
#1Vikash Kumar Shah (CBNU: Chungbuk National University)H-Index: 3
#2Jae Joon Choi (CBNU: Chungbuk National University)H-Index: 3
Last. Ki-Wan Oh (CBNU: Chungbuk National University)H-Index: 31
view all 6 authors...
This study was investigated to know whether pachymic acid (PA), one of the predominant triterpenoids in Poria cocos (Hoelen) has the sedative-hypnotic effects, and underlying mechanisms are mediated via γ-aminobutyric acid (GABA)-ergic systems. Oral administration of PA markedly suppressed locomotion activity in mice. This compound also prolonged sleeping time, and reduced sleep latency showing synergic effects with muscimol (0.2 mg/kg) in shortening sleep onset and enhancing sleep time induced ...
21 CitationsSource
#1Bing Xia (CAS: Chinese Academy of Sciences)H-Index: 13
#2Yan Zhou (CAS: Chinese Academy of Sciences)H-Index: 27
Last. Hong-Xi Xu (SHU: Shanghai University)H-Index: 71
view all 5 authors...
A sensitive, precise and accurate method was developed to screen and quantify triterpenoids based on ultra-performance liquid chromatography-photodiode array-quadrupole time-of-flight mass spectrometry (UPLC-PDA-QTOE-MS). An exact neutral loss scan of 62.0004 Da (CH2O3) was used to selectively detect triterpenoids in Poria cocos, followed by a survey scan for exact masses of precursor and fragment ions of these triterpenoids. The developed method was applied to quantify seven major triterpenoids...
23 CitationsSource
#1Jin-Woo Jeong (DGIST: Daegu Gyeongbuk Institute of Science and Technology)H-Index: 21
#2Hye Hyeon LeeH-Index: 8
Last. Yung Hyun ChoiH-Index: 75
view all 7 authors...
Background Poria cocos Wolf, a medicinal fungus, is widely used in traditional medicines in East Asian countries owing to its various therapeutic potentials. Although several studies have demonstrated the anti-inflammatory activity of this fungus, its underlying mechanisms have not yet been clearly defined.
30 CitationsSource
#1Ya-Long Feng (MOE: Chinese Ministry of Education)H-Index: 18
#2Ping LeiH-Index: 2
Last. Rui-Chao Lin (FDA: Food and Drug Administration)H-Index: 29
view all 9 authors...
Abstract Ethnopharmacological relevance Poria cocos Wolf (Polyporaceae) is a well-known medicinal fungus, the epidermis (“Fu-Ling-Pi” in Chinese) of the sclerotia is used as a diuretic for treating oedema and promoting the diuretic process. In this paper we report on the diuretic activity in rats of petroleum ether, ethyl acetate, n-butanol and the remaining fractions of the ethanol extract from the epidermis of Poria cocos . Materials and methods Petroleum ether, ethyl acetate, n-butanol and th...
63 CitationsSource
#1Yuanzhong WangH-Index: 19
Last. Honggao Liu (Yunnan Agricultural University)H-Index: 15
view all 8 authors...
Abstract Ethnopharmacological relevance Wolfiporia cocos (Schwein.) Ryvarden et Gilb. has a long history as a Chinese traditional medicine with uses of inducing diuresis, excreting dampness, invigorating the spleen, and tranquilizing the mind. Recently, Wolfiporia cocos has received increasing interest, and phytochemical and pharmacological studies have validated the traditional uses of this species. Aims of the review To provide an up-to-date and comprehensive overview of the mycology, cultivat...
84 CitationsSource
Cited By20
#1Hui Jiang (Anhui University of Chinese Medicine)
#2Liu Jian (Anhui University of Chinese Medicine)
Last. Wang Bin (Anhui University of Chinese Medicine)
view all 7 authors...
The appropriate selection of quality marker (Q-marker) for performing the comprehensive quality evaluation of traditional Chinese medicines (TCMs) has much more significance. Wu-Wei-Wen-Tong Capsule (WWWTC), a TCMs prescription, is mainly utilized to treat rheumatoid arthritis (RA) in China. However, the comprehensive quality control for WWWTC has not been achieved because of lacking system analysis for the Q-marker. In this study, a dual wavelength, 203 and 270 nm, was selected based on the fea...
#1Binbin Wang (Capital Medical University)
#2Xiaoxiao Feng (Capital Medical University)
Last. Zhaoxia Li (Capital Medical University)H-Index: 6
view all 6 authors...
Kaixin Powder (KXP) is a classic formula for treating morbid forgetfulness in ancient China. To guarantee the efficacy and safety of KXP, a simple and accurate HPLC–DAD method has been established ...
#1Yumeng Zhang (SPU: Shenyang Pharmaceutical University)H-Index: 4
#2Min Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 8
Last. Miao Wang (SPU: Shenyang Pharmaceutical University)H-Index: 9
view all 6 authors...
Abstract Yinchen Wuling Powder (YCWLP) is a classic Chinese medicine prescription with a long history and has been commonly used for treating jaundice hepatitis, liver fibrosis, hyperlipidemia and early diabetes in clinical applications. However, the chemical composition of YCWLP is still unclear. In order to obtain the chemical profile of YCWLP, a systematic ultra-performance liquid chromatography coupled with fourier transform ion cyclotron resonance mass spectrometry (UPLC-FT-ICR-MS) method w...
#1Feng Liu (HAU: Huazhong Agricultural University)H-Index: 1
#2Ying Liu (HAU: Huazhong Agricultural University)H-Index: 19
Last. Wen Huang (HAU: Huazhong Agricultural University)H-Index: 11
view all 5 authors...
Abstract Carboxymethyl pachymaran (CMP) was prepared from Poria cocos polysaccharide by carboxymethylation. Two types of CMP (CMP-1 and CMP-2) were further purified by DEAE-52 anion-exchange chromatography. The structure characteristics and immunomodulatory activities of CMP-1 and CMP-2 were investigated. CMP-1 was determined as β-(1 → 3)- d -glucan. A β-(1 → 3)- d -glucan backbone structure was also found in CMP-2, which was mainly consistent of mannose and glucose, with the mole ratio of 0.03:...
#1Hongmei Luo (Peking Union Medical College)H-Index: 28
#2Jun Qian (Peking Union Medical College)H-Index: 17
Last. Shilin Chen (Peking Union Medical College)H-Index: 64
view all 16 authors...
Abstract Wolfiporia cocos (F. A. Wolf) has been praised as a food delicacy and medicine for centuries in China. Here, we present the genome and transcriptome of the Chinese strain CGMCC5.78 of W. cocos. High-confidence functional prediction was made for 9277 genes among the 10,908 total predicted gene models in the W. cocos genome. Up to 2838 differentially expressed genes (DEGs) were identified to be related to sclerotium development by comparing the transcriptomes of mycelial and sclerotial ti...
#1Anzheng Nie (Zhengzhou University)H-Index: 3
#2Yanhui Chao (Zhengzhou University)H-Index: 1
Last. Chunsheng Zhu (Zhengzhou University)H-Index: 29
view all 6 authors...
Poria cocos is the dried sclerotium of Wolfiporia cocos (F.A. Wolf) Ryvarden & Gilb., which was the current accepted name and was formerly known as Macrohyporia cocos (Schwein.) I. Johans. & Ryvarden, Pachyma cocos (Schwein.) Fr., Poria cocos F.A. Wolf and Sclerotium cocos Schwein. It is one of the most important crude drugs in traditional Chinese medicine, with a wide range of applications in ameliorating phlegm and edema, relieving nephrosis and chronic gastritis and improving uneasiness of mi...
3 CitationsSource
#1Bo Liang (Nanjing University of Chinese Medicine)H-Index: 1
#1Bo Liang (Nanjing University of Chinese Medicine)H-Index: 5
Last. Hui-Ling LiaoH-Index: 2
view all 4 authors...
Background. Dingji Fumai decoction (DFD) is used to treat ventricular arrhythmia, and it has provided a very good curative effect. However, its cellular electrophysiological mechanism is unknown. Methods. Electrocardiogram was recorded, and oxidative stress response and ion-channel-related molecules were detected in rats with barium chloride- and aconitine-induced ventricular arrhythmia. Moreover, whole-cell patch-clamp assay was used to investigate the inhibitory effect of DFD on Nav1.5 in Chin...
2 CitationsSource
Abstract Dried sclerotium of Macrohyporia cocos is a well-known and widely-consumed traditional Chinese medicine and is also used as dietary supplement. According to the differential treatment between cultivation and wild habitats in the market, the comparison and quantitative analysis of wild and cultivated M. cocos were performed using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy and ultra-fast liquid chromatography combined with partial least squares discrim...
2 CitationsSource
#1Lixia Zhu (Hong Kong Baptist University)H-Index: 6
#2Xu WangH-Index: 1
Last. Zhongzhen Zhao (Hong Kong Baptist University)H-Index: 50
view all 10 authors...
Abstract Poria cocos (Schw.) Wolf has been widely used in traditional Chinese medicine (TCM) for centuries. Its three medicinal parts are Poria Cutis, the epidermis or fulingpi in Chinese; White Poria, the middle part or baifuling; and Poria cum Radix Pini, the sclerotium with some part of host pine root or fushen. The hostwood in fushen is the inner part, known as fushenmu. The epidermis, middle part and middle-plus-inner part have different clinical applications, but the differences in their c...
5 CitationsSource
#1Yuqi Mei (Nanjing University of Chinese Medicine)H-Index: 5
#2Lifang Wei (Nanjing University of Chinese Medicine)H-Index: 5
Last. Shengxin YinH-Index: 3
view all 11 authors...
Spatholobi Caulis (SC), the vine stem of Spatholobus suberectus Dunn, is a widely used traditional Chinese medicine (TCM) for the treatment of blood stasis syndrome and related diseases. Xylem and phloem are the main structures of SC and the color of xylem in SC is red brown or brown while the phloem with resin secretions is reddish brown to dark brown. They are alternately arranged in a plurality of concentric or eccentric rings. In order to investigate the distribution patterns of metabolites ...
4 CitationsSource