A high throughput method to study the physiology of E2:ERα signaling in breast cancer cells

17β‐estradiol (E2) regulates diverse physiological effects including cell proliferation through the estrogen receptor α (ERα), which as a transcription factor drives gene transcription and as an extra‐nuclear localized receptor triggers the membrane‐dependent activation of diverse kinase cascades. E2 also modifies ERα intracellular levels via diverse intracellular mechanisms. In this way, the E2‐acivated ERα integrates signaling cascades with...