Potential testing of reprocessing procedures by real-time polymerase chain reaction: A multicenter study of colonoscopy devices

Reprocessing of endoscopes is key to preventing cross-infection after colonoscopy. Culture-based methods are recommended for monitoring, but alternative and rapid approaches are needed to improve surveillance and reduce turnover times. A molecular strategy based on detection of residual traces from gut microbiota was developed and tested using a multicenter survey.A simplified sampling and DNA extraction protocol using nylon-tipped flocked swabs...