Investigation of the preventive effect of Sijunzi decoction on mitomycin C-induced immunotoxicity in rats by 1H NMR and MS-based untargeted metabolomic analysis.
Abstract Ethnopharmacological relevance Sijunzi decoction (SJZD) is a well known traditional Chinese prescription used for the treatment of gastrointestinal disorders and immunity enhancement. It has been found to indeed improve life quality of chemotherapy patients and extensive used in clinical conbined with chemotherapeutics for the treatment of cancer. Aim of the study The aim of this study was to investigate the preventive effect of the immunotoxicity of SJZD on mitomycin C (MMC) and the metabolic mechanism of action. Materials and methods NMR and MS-based metabolomics approaches were combined for monitoring MMC-induced immunotoxicity and the protective effect of SJZD. Body weight change and mortality, histopathological observations and relative viscera weight determinations of spleen and thymus, sternum micronucleus assay and hematological analysis were used to confirm the immunotoxicity and attenuation effects. An OPLS-DA approach was used to screen potential biomarkers of immunotoxicity and the MetaboAnalyst and KEGG PATHWAY Database were used to investigate the metabolic pathways. Results 8 biomarkers in plasma samples, 19 in urine samples and 10 in spleen samples were identified as being primarily involved in amino acid metabolism, carbohydrate metabolism and lipid metabolism. The most critical pathway was alanine, aspartate and glutamate metabolism. Conclusions The variations in biomarkers revealed the preventive effect of the immunotoxicity of SJZD on MMC and significant for speculating the possible metabolic mechanism.
1, 1, 2-Trichloroethylene (TCE) is of environmental concern, due to evaporation while handling, chemical processing and leakage from chemical waste sites, leading to its contamination of ground water and air. For several decades there has been issues about possible long term health effects of TCE but recently the International Agency for Research on Cancer (IARC) and the US Environmental Protection Agency classified TCE as a human carcinogen. Links having been established between occupational ex...
Cyclophosphamide (CY) is the most commonly used antineoplastic drug, but various side effects are often observed. In this work, a 1H NMR based metabolomic approach combined with biochemical assay and histopathological inspection were employed to study the toxic effects of CY on rats. The endogenous metabolites in liver, kidney, and heart were identified. Multivariate analysis revealed that the 18 metabolites in the liver, 14 metabolites in the kidney, and 6 metabolites in the heart were signific...
Metabolites are building blocks of cellular function. These species are involved in enzyme-catalyzed chemical reactions and are essential for cellular function. Upstream biological disruptions result in a series of metabolomic changes and, as such, the metabolome holds a wealth of information that is thought to be most predictive of phenotype. Uncovering this knowledge is a work in progress. The field of metabolomics is still maturing; the community has leveraged proteomics experience when appli...
Abstract Background Recurrent complex esophageal strictures remain difficult to manage. Aims To determine the efficacy of topical Mitomycin C application for recurrent benign esophageal strictures. Methods All patients who underwent balloon dilation followed by topical Mitomycin C application for recurrent benign esophageal strictures were included. Primary outcome was number of dilations and change of dysphagia score. Results Nine patients with anastomotic (3), radiation-induced (3), caustic (2...
AbstractObjective: The aim of this study was to compare the long-term efficacy of BCG monotherapy to alternating therapy of mitomycin C (MMC) and BCG in patients with carcinoma in situ (CIS).Materials and methods: Between 1992 and 1997, 321 patients with CIS were randomized from Finland, Norway and Sweden in a prospective multicenter trial into two treatment groups. The alternating therapy comprised six weekly instillations of MMC 40 mg followed by 10 instillations of BCG (Connaught 120 mg) or M...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 6
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Abstract Ethnopharmacological relevance Cortex Fraxini (CF) is an important traditional Chinese herbal medicine used for the treatment of gout and hyperuricemia. Aim of the study The aim of this study was to evaluate the anti-hyperuricemic effect of CF on hyperuricemic rats and to investigate its mechanism of action. Materials and methods Metabolomics based on NMR and MS was used to study the therapeutic effect of CF on hyperuricemic rats. Plasma determination of uric acid (UA) showed that CF tr...
Introduction Anticancer treatment results in temporary or permanent toxicity considered as changes in normal tissues and/or involved regions. The net effect is mirrored in morphological, functional and molecular disturbances—thus in a systemic response of the human body. To date, specific NMR biomarkers of radiation therapy toxicity in head and neck squamous cell carcinoma (HNSCC) patients are scarce or even missing.
Last. Gary Siuzdak(Scripps Research Institute)H-Index: 88
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Metabolomics has been utilized extensively for the identification of single metabolites and their use as biomarkers. Owing to recent technical advances, it is now possible to use metabolomics to better understand whole metabolic pathways and to more precisely pinpoint the involvement of metabolites in physiology and pathology.
Metabolomics can be performed either as an ‘open profiling’ tool where the aim is to measure, usually in a semi-quantitative manner, as many metabolites as possible or perform ‘closed’ or ‘targeted’ analyses where instead a pre-defined set of metabolites are measured. Targeted methods can be designed to be more sensitive and quantitative and so are particularly appropriate to systems biology for quantitative models of systems or when metabolomics is performed in a hypothesis driven manner to tes...
#1Emmy Y. Li(CUHK: The Chinese University of Hong Kong)H-Index: 13
#2Andy C. O. Cheng(CUHK: The Chinese University of Hong Kong)H-Index: 10
Last. Hunter K.L. Yuen(CUHK: The Chinese University of Hong Kong)H-Index: 11
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One of the common causes of failure in dacryocystorhinostomy for nasolacrimal duct obstruction (NLDO) is mucosal scarring and fibrosis around the ostium. Steroid and mitomycin C (MMC) can potentially reduce scarring by their action on the inflammatory and proliferative phase of wound healing, respectively. The purpose of this study is to evaluate the safety and efficacy of combined usage of adjunctive MMC and intranasal triamcinolone (TA) in endonasal endoscopic dacryocystorhinostomy (EE-DCR). T...
Last. Qiqing Zhang(Xinxiang Medical University)H-Index: 53
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Abstract A classic prescription in traditional Chinese medicine, Sijunzi decoction is widely used to treat spleen deficiency syndrome. However, the therapeutic mechanisms are not yet well understood. In this study, the therapeutic effects of Sijunzi decoction were assessed by histopathological examination and enzyme activity analysis (succinate dehydrogenase (SDH) and lactic dehydrogenase (LDH)). A label-free proteomics method was used to assess the changes in the endogenous protein and to explo...
Last. Yunli Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 16
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Abstract A metabonomic approach involving an ultrahigh-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) was used to investigate the changes in the endogenous metabolites in the plasma of rats with yeast-induced pyrexia treated with Gegenqinlian decoction (GQLD), aspirin and itraconazole. The differences in the small molecule profiles of treatment using Traditional Chinese medicine, etiological treatment and symptomatic ...
#1Yuqi Fan(Tianjin University of Traditional Chinese Medicine)H-Index: 2
#2Zhe Ma(Tianjin University of Traditional Chinese Medicine)H-Index: 4
Last. Jun He(Tianjin University of Traditional Chinese Medicine)H-Index: 22
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Cancer, the leading cause of death in the world nowadays, is a major public health problem. The therapeutic efficacy of cancer is still unsatisfactory despite the development of various treatments. Traditional Chinese medicine (TCM) formulas are a vital therapeutic strategy and new treatment approach for cancer, as they exhibit prospective antitumor potential with multiple targets, multiple signaling pathways and less side effects. In this review, the anticancer functions of a variety of TCM for...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 6
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Shaoyao Gancao decoction (SGD) is a famous Chinese traditional prescription for treating liver injury. In this research, we investigated the liver protective effects of SGD and its metabolic mechanisms by 1 H-NMR and UPLC-MS. Serum biochemical indicators and histopathological methods werae used to identify the status of SGD in treating liver injury. An OPLS-DA method was used to screen potential differential metabolic markers, and the MetaboAnalyst and KEGG PATHWAY databases were used to find re...
#1Qinghua Yan(Xinxiang Medical University)H-Index: 1
#2Huili Mao(Xinxiang Medical University)H-Index: 1
Last. Jianyi Guan(Xinxiang Medical University)H-Index: 1
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Abstract Sijunzi decoction (SJZD) is a traditional Chinese medicine prescription which is used to treat spleen deficiency syndrome. Its mechanism of action was studied in a rat model of reserpine-induced spleen deficiency syndrome with metabolomics assays performed in normal control, model and Sijunzi decoction treated rats by liquid chromatography quadruple-time-of-flight mass spectrometry (LC-Q/TOF-MS). Enzyme assays and histopathology, with multivariate statistical analysis, showed that Sijun...
: Semen descurainiae oil (SDO) is an important traditional Chinese medicine that was recently discovered to have the function of reducing blood lipids. Metabolomics analyses of plasma, liver and kidney in rats were performed using 1 H-NMR and LC-MS to illuminate the lower blood lipid concentration effect of SDO, and niacin was considered as the active control. The measure of total cholesterol (TC) and low-density-lipoprotein cholesterol (LDL-C) in plasma showed that SDO treatment decreased signi...
#1Xiaowei Zhou(Peking Union Medical College)H-Index: 2
#2Ying Li(Peking Union Medical College)H-Index: 31
Last. Guohui Li(Peking Union Medical College)H-Index: 5
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Nowadays, an increasing number of patients are seriously affected by lung cancer. Si Jun Zi Tang (SJZ), a four-herb Chinese medicine formula first described approximately one thousand years ago, is often prescribed for cancer patients as a complementary therapy. But the research on the effective materials for treating cancer using SJZ was rarely reported. To solve this problem, we evaluate the inhibitory effect of 10 samples of SJZ from different origins on PC9 cells. Ultraperformance liquid chr...
Last. Tingli Qu(Shanxi Medical University)H-Index: 3
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ABSTRACTThis study aims to provide a scientific basis for the comprehensive utilization of the aerial parts of Astragalus membranaceus (APAM). UPLC-Qtrap HRMS was used to identify secondary metabol...
Last. Jin-Mei Lai(FJU: Fu Jen Catholic University)H-Index: 11
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Abstract Ethnopharmacological relevance Sijunzi decoction is a well-known traditional Chinese medicine (TCM) commonly used for invigorating vital energy and for the enhancement of immunity. Modified Sijunzi decoctions have been extensively used to treat cachexia and improve the quality of life of cancer patients undergoing chemotherapy. Aim of the study This study was aimed to provide comprehensive evidence for the anti-cachectic effect of a modified Sijunzi decoction (Zhen-Qi; ZQ-SJZ) and chara...
Last. Guohui Li(Peking Union Medical College)H-Index: 5
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Abstract Lung cancer remains the leading cause of cancer death worldwide, and the current therapy seems to have reached a plateau due to toxicities and acquired resistance. Therefore, exploration of novel therapeutic avenues may be useful. Si Jun Zi Tang (SJZ), a four-herb Chinese medicine formula first described approximately one thousand years, is often prescribed for cancer patients as a complementary therapy. However, whether SJZ benefits cancer patients as well as the main active constituen...