Identification of bioactive compounds in Shaoyao-Gancao decoction using β2-adrenoceptor affinity chromatography.

Published on Jun 1, 2017in Journal of Separation Science2.878
· DOI :10.1002/JSSC.201700113
Zehua Li1
Estimated H-index: 1
(Northwest University (United States)),
Haiyang Gao1
Estimated H-index: 1
(Northwest University (United States))
+ 1 AuthorsYajun Zhang1
Estimated H-index: 1
(Northwest University (United States))
Sources
Abstract
Shaoyao-Gancao decoction, a Chinese herbal formula, is composed of Paeoniae Radix alba and Glycyrrhiza Radix et rhizoma. It has been widely used to treat muscle spasms and asthma. However, little is known about the bioactive components of Shaoyao-Gancao decoction. In the present study, the bioactive compounds in water-extract of Shaoyao-Gancao decoction were separated by the immobilized β2 -adrenoceptor affinity column and identified using quadrupole time-of-flight mass spectrometry. The affinity constants of the separated compounds that bind to β2 -adrenoceptor were determined by frontal analysis. Compound bioactivity was tested in a rat tracheal smooth muscle relaxation assay. We identified the bioactive compounds in the water extract of Shaoyao-Gancao decoction that bound to the β2 -adrenoceptor as paeoniflorin and liquiritin. Paeoniflorin and liquiritin had only one binding site on the immobilized β2 -adrenoceptor, and the affinity constants were (2.16 ± 0.10) × 104  M-1 and (2.95 ± 0.15) × 104  M-1 , respectively. Both compounds induced a concentration-dependent relaxation of tracheal smooth muscle following K+ -stimulated contraction, and the relaxation effects were abrogated by the β2 -adrenoceptor antagonist, ICI 118551. Therefore, paeoniflorin and liquiritin are bioactive compounds in Shaoyao-Gancao decoction and the β2 -adrenoceptor affinity chromatography is a useful tool for identifying potential β2 -adrenoceptor ligands in natural products used in traditional Chinese medicine.
📖 Papers frequently viewed together
20123.27Molecules
5 Authors (Yan Chen, ..., Qingqing Wu)
18 Citations
7 Citations
74 Citations
References26
Newest
#1Yajun Zhang (MOE: Chinese Ministry of Education)H-Index: 8
#2Kaizhu Zeng (MOE: Chinese Ministry of Education)H-Index: 4
Last. Xiaohui Zheng (MOE: Chinese Ministry of Education)H-Index: 15
view all 6 authors...
As a xanthine derivative, doxofylline is believed to be dominant for fighting against asthma in practice. Unlike other xanthines, the antiasthmatic effects of doxofylline lack any definite proof of target and mediating mechanism according to previous reports. In this work, the interaction between doxofylline and β2 -AR was investigated by high performance affinity chromatography using frontal analysis and nonlinear model. The methodology involved the immobilization of β2 -AR on the silica gel by...
10 CitationsSource
#1Huihua Qu (Beijing University of Chinese Medicine)H-Index: 10
#2Yue Zhang (Beijing University of Chinese Medicine)H-Index: 8
Last. Yan Zhao (Beijing University of Chinese Medicine)H-Index: 8
view all 8 authors...
In this work, a novel monoclonal antibody specific for naringin was prepared and characterized. Subsequently, an indirect competitive enzyme-linked immunosorbent assay for naringin was developed, with an effective range from 4.8 to 156 ng/mL naringin. Next, an immunoaffinity column was obtained by coupling anti-naringin monoclonal antibodies to CNBr-activated Sepharose 4B and a rapid immunoaffinity chromatography assay for naringin was developed. The immunoaffinity column was used to separate na...
5 CitationsSource
#1Haiyang Gao (Northwest University (United States))H-Index: 1
#2Zhe Sun (Northwest University (United States))H-Index: 2
Last. Yajun Zhang (Northwest University (United States))H-Index: 8
view all 5 authors...
Shaoyao-Gancao decoction (SGD), a traditional Chinese herbal medicine, was originally described in the Treatise on Cold Pathogenic Diseases and is composed of Paeoniae Radix alba and Glycyrrhiza Radix et rhizoma. It has been widely used in treating spasmolysis and asthma. In the present study, the therapeutic effects of SGD were assessed, and the detailed mechanisms of SGD for asthma treatment were investigated. We established a rat asthma model and analyzed the changes in the metabonomic profil...
5 CitationsSource
#1May Bakail (Université Paris-Saclay)H-Index: 2
#2Françoise Ochsenbein (Université Paris-Saclay)H-Index: 18
Abstract Targeting protein–protein interactions has long been considered as a very difficult if impossible task, but over the past decade, front lines have moved. The number of successful examples is exponentially growing. This review presents a rapid overview of recent advances in this field considering the strengths and weaknesses of the small molecule approaches and alternative strategies such as the selection or design of artificial antibodies, peptides or peptidomimetics.
63 CitationsSource
: As the considerable technical challenges involved with generating crystal structures of G (guanine nucleotide- binding) protein-coupled receptors (GPCRs) are starting to be successfully addressed, opportunities to apply fragment-based drug discovery (FBDD) to this class of target are becoming a reality. GPCRs represent a large and important family of drug targets with considerable clinical and commercial interest. While their general seven transmembrane helix bundle structures are amenable to ...
3 CitationsSource
#1Mina Salary (University of Mazandaran)H-Index: 3
#2Mohammad Reza Hadjmohammadi (University of Mazandaran)H-Index: 24
Abstract Human serum albumin (HSA) is the most important drug carrier in humans mainly binding acidic drugs. Negatively charged compounds bind more strongly to HSA than it would be expected from their lipophilicity alone. With the development of new acidic drugs, there is a high need for rapid and simple protein binding screening technologies. Biopartitioning micellar chromatography (BMC) is a mode of micellar liquid chromatography, which can be used as an in vitro system to model the biopartiti...
10 CitationsSource
#1Tao Zhang (Xi'an Jiaotong University)H-Index: 15
#2Yuanyuan Ding (Xi'an Jiaotong University)H-Index: 7
Last. Sicen Wang (Xi'an Jiaotong University)H-Index: 23
view all 5 authors...
Tyrosine 367 Cysteine-fibroblast growth factor receptor 4 cell membrane chromatography combined with high-performance liquid chromatography and mass spectrometry was developed. Tyrosine 367 Cysteine-HEK293 cells were used as the cell membrane stationary phase. The specificity and reproducibility of the cell membrane chromatography was evaluated using 1-tert-butyl-3-{2-[4-(diethylamino)butylamino]-6-(3,5-dimethoxyphenyl)pyrido[2,3-d]pyrimidin-7-yl}urea, nimodipine and dexamethasone acetate. Then,...
14 CitationsSource
#1Tianzhu Zhang (Changchun University)H-Index: 5
#2Zhaocong Yang (Nanjing University of Chinese Medicine)H-Index: 1
Last. Shumin Wang (Changchun University)H-Index: 4
view all 5 authors...
Paeoniflorin has been demonstrated to exert anti-inflammatory and immunomodulatory effects in the animal study. In this study, we investigated immunoregulatory effects of paeoniflorin on anti-asthmatic effects and underlying mechanisms. Asthma model was established by ovalbumin-induced. A total of 50 mice were randomly assigned to five experimental groups: control, model, dexamethasone (2 mg/kg), and paeoniflorin (10 and 20 mg/kg). Airway resistance (Raw) were measured by the forced oscillation ...
19 CitationsSource
#1Jerome Hochman (MSD: Merck & Co.)H-Index: 14
#2Cuyue TangH-Index: 26
Last. Thomayant Prueksaritanont (MSD: Merck & Co.)H-Index: 44
view all 3 authors...
ABSTRACT: Drug–drug interactions (DDIs) related to altered drug absorption and plasma protein binding have received much less attention from regulatory agencies relative to DDIs mediated via drug metabolizing enzymes and transporters. In this review, a number of theoretical bases and regulatory framework are presented for these DDI aspects. Also presented is an industry perspective on how to approach these issues in support of drug development. Overall, with the exception of highly permeable and...
16 CitationsSource
#1Jing Sun (Fudan University)H-Index: 15
#2Jinfeng Wu (Fudan University)H-Index: 22
Last. Jingcheng Dong (Fudan University)H-Index: 26
view all 6 authors...
Abstract Paeoniflorin (PF), one of the major active ingredients of Chinese peony , has demonstrated anti-inflammatory and immunoregulatory effects. However, it has remained unclear whether PF treatment can inhibit allergic inflammation in asthma. In this study, we evaluated the effects of PF on pulmonary function and airway inflammation in asthmatic mice. The allergic asthma models were established in BALB/c mice. The mice were sensitized and challenged with ovalbumin. Airway hyperresponsiveness...
26 CitationsSource
Cited By11
Newest
#1Jianwei Dou (Xi'an Jiaotong University)H-Index: 4
#2Zhongbo ZhuH-Index: 1
Last. Jianyu He (Xi'an Jiaotong University)H-Index: 2
view all 9 authors...
Yanghe decoction is a traditional Chinese medicine prescription and has been used for breast cancer treatment for many years. However, the effective ingredients in the decoction have not been identified. The expression of poly(ADP-ribose) polymerase-1 is highly related to breast cancer. Using poly(ADP-ribose) polymerase-1 as a probe, we expressed the haloalkane dehalogenase-tagged protein in BL21(DE3) E. coli, immobilized it on hexachlorocaproic acid-modified macroporous silica gel, and establis...
Source
#1Lin Sun (SPU: Shenyang Pharmaceutical University)H-Index: 1
#2Min Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 8
Last. Chunjie Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 6
view all 7 authors...
Shaoyao Gancao decoction (SGD), a well-known Chinese herbal formula, has been used to treat liver injury for a long time. In this study, chemical profiles of SGD were identified using ultra high-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS/MS). Liquid chromatography was performed on a C18 column (150 mm × 2.1 mm, 1.8 μm); the mobile phase comprised 0.1% formic acid (A) and acetonitrile (B). We then characterized 73 c...
3 CitationsSource
#1Dan Wang (SHU: Shanghai University)H-Index: 2
#2Hao Zhao (SHU: Shanghai University)
Last. Dong-Hui Wei (SHU: Shanghai University)
view all 5 authors...
OBJECTIVE To investigate the mechanism of cAMP-PKA signaling pathway mediated by Chinese medicine formula Shaoyao Gancao Decoction (, SGD) on the regulation of aquaporin 5 (AQP5) and muscarinic receptor 3 (M3R) levels in Sjogren's syndrome (SS). METHODS Of the 30 mice, 5 were randomly selected as control, and others were used for creating SS model. After successful modeling, mice were randomly divided into 5 groups (n=5 per group) and intragastrically administered with saline (8 mL/kg), pilocarp...
2 CitationsSource
#1Marcela Cristina de Moraes (Federal Fluminense University)H-Index: 10
#2Carmen Lúcia Cardoso (FFCLRP: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto)H-Index: 18
Last. Quezia B. Cass (UFSCar: Federal University of São Carlos)H-Index: 35
view all 3 authors...
Ligand-target interactions play a central role in drug discovery processes because these interactions are crucial in biological systems. Small molecules-proteins interactions can regulate and modulate protein function and activity through conformational changes. Therefore, bioanalytical tools to screen new ligands have focused mainly on probing ligand-target interactions. These interactions have been evaluated by using solid-supported proteins, which provide advantages like increased protein sta...
7 CitationsSource
#1Jun-jin Liu (Shanxi Medical University)H-Index: 3
#2Yao Cheng (Shanxi Medical University)H-Index: 3
Last. Rui-gang Hou (Shanxi Medical University)H-Index: 3
view all 10 authors...
: A simple and sensitive liquid chromatography with tandem mass spectrometry method was developed for simultaneous quantification of paeoniflorin, albiflorin, oxypaeoniflorin, liquiritin, liquiritigenin, glycyrrhetinic acid, and glycyrrhizin in rat plasma after oral administration of Shaoyao-Gancao decoction, which is traditionally used in the treatment of polycystic ovary syndrome. The plasma samples were pretreated with methanol as precipitant. The method exhibited good linearity (correlation ...
5 CitationsSource
#1Hao Zhang (Chongqing University)H-Index: 6
#2Zhao-Yu Wu (Chongqing University)H-Index: 4
Last. Shao-Ping Li (UM: University of Macau)H-Index: 45
view all 5 authors...
Abstract Immobilization of biomaterials developed rapidly due to the great promise in improving their stability, activity and even selectivity. In this review, the immobilization strategies of biomaterials, including physical adsorption, encapsulation, covalent attachment, cross-linking and affinity linkage, were briefly introduced. Then, the major emphasis was focused on the reported various types of immobilized biomaterials, including proteins, enzymes, cell membrane and artificial membrane, l...
8 CitationsSource
#1Jialiang Guo (FOSU: Foshan University)H-Index: 12
#2Hang Lin (JNU: Jinan University)H-Index: 3
Last. Zhengjin Jiang (JNU: Jinan University)H-Index: 26
view all 6 authors...
Abstract Natural products are one of the most important sources of leading compounds in drug discovery. Until now, rapid screening and identification of biologically active components from natural products remain a technical challenge. Bio-affinity chromatography (BAC) based approaches hold great promise in screening potential bioactive ligands from complex samples. In this review, we summarized recent development of BAC in natural products screening, with a particular emphasis on the reported b...
14 CitationsSource
#1Man Zhu (Xi'an Jiaotong University)H-Index: 8
#2Yuxin Cui (Xi'an Jiaotong University)H-Index: 1
Last. Yanmin Zhang (Xi'an Jiaotong University)H-Index: 34
view all 8 authors...
Abstract Erythropoietin-producing hepatocyte B4 (EphB4) has recently been reported to be an oncogenic factor in many cancers and overexpressed in several types of tumors. Thus, EphB4 has the potential to be a therapeutic target in these malignancies. High-performance affinity chromatography has been a powerful tool to study the interaction between drugs and receptors. In this study, we constructed a novel EphB4 affinity chromatography model to investigate the active compounds which can target Ep...
2 CitationsSource
#1Fuhuan Fei (MOE: Chinese Ministry of Education)H-Index: 3
#2Huanmei Sun (MOE: Chinese Ministry of Education)H-Index: 3
Last. Yajun Zhang (MOE: Chinese Ministry of Education)H-Index: 8
view all 7 authors...
San-ao decoction (SAD) has been widely used in Chinese medicine against respiratory diseases, such as asthma and rhinallergosis. The bioactive compounds for such pharmacological action remain unknown. We developed a methodology to isolate the bioactive compounds of SAD. The assay involved the immobilization of beta 2-adrenoceptor (β2-AR) onto magnetic fine particles, the capture of target compounds by the immobilized receptor, the identification of the receptor bound compounds by reversed-phase ...
2 CitationsSource
#1Xiaoshuang He (Xi'an Jiaotong University)H-Index: 5
#2Yue Sui (Xi'an Jiaotong University)H-Index: 2
Last. Sicen Wang (Xi'an Jiaotong University)H-Index: 23
view all 3 authors...
Frontal affinity chromatography is an efficient technique that combines affinity interaction and high-performance liquid chromatography, and frontal analysis has been used in studying the interaction between drugs and proteins. Based on frontal analysis, stepwise frontal analysis has been established. The present study aimed to use the Lineweaver–Burk plot in stepwise frontal analysis by taking the weighted average of time data. Commercial human serum albumin (HSA) and alpha-1-acid glycoprotein ...
5 CitationsSource