Original paper
Development of the technique for isolation and primary culture of bovine hepatocytes.
Abstract
Collagenase perfusion method was used to isolate the bovine hepatocytes of caudate lobe obtained from a neonatal calf serum factory.Total cell count and survival rate of freshly isolated hepatocytes were determined by trypan blue exclusion.Hepatocytes were seeded onto a cattle tail collagen coated 6-well microplate at a density of 1×106 cells/well.Morphological assessment of hepatocyte monolayers and determination of LDH activity in the culture...
Paper Details
Title
Development of the technique for isolation and primary culture of bovine hepatocytes.
Published Date
Jan 1, 2009
Journal
Volume
29
Issue
2
Pages
203 - 206
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Notes
History