Development of the technique for isolation and primary culture of bovine hepatocytes.

Collagenase perfusion method was used to isolate the bovine hepatocytes of caudate lobe obtained from a neonatal calf serum factory.Total cell count and survival rate of freshly isolated hepatocytes were determined by trypan blue exclusion.Hepatocytes were seeded onto a cattle tail collagen coated 6-well microplate at a density of 1×106 cells/well.Morphological assessment of hepatocyte monolayers and determination of LDH activity in the culture...