ZNF503/Zpo2 drives aggressive breast cancer progression by down-regulation of GATA3 expression.

Published on Mar 21, 2017in Proceedings of the National Academy of Sciences of the United States of America9.412
· DOI :10.1073/PNAS.1701690114
Payam Shahi12
Estimated H-index: 12
(UCSF: University of California, San Francisco),
Chih Yang Wang16
Estimated H-index: 16
(UCSF: University of California, San Francisco)
+ 6 AuthorsZena Werb179
Estimated H-index: 179
(UCSF: University of California, San Francisco)
Sources
Abstract
Effect of elevated Zpo2 levels on Zpo1-, GATA3-, and EMT-associated genes. (A) qRT-PCR analysis indicating GATA3 levels in EpH4.9 cells overexpressing Zpo1 and Zpo2. (B) qRT-PCR analysis indicating that shRNA-mediated down-regulation of Zpo2 increases Gata3 levels. (C) Western blot analysis in EpH4.9 mammary epithelial cell extracts. Zpo2 overexpression lowers GATA3 levels. Zpo1 overexpression does not alter GATA3 levels. Down-regulation of Zpo2 elevates GATA3 levels. (D) qRT-PCR analysis of Gata3 expression in PyMT cells overexpressing Zpo2. Zpo2 down-regulates Gata3 levels. (E) qRT-PCR analysis in PyMT cells. Down-regulation of Zpo2 results in increased Gata3 levels. (F) qRT-PCR analysis of EMT-associated genes in PyMT cells. Overexpression of Zpo2 alters EMT-associated gene expression (G) Zymogram analysis indicating increased MMP9 levels in EpH4.9 mammary epithelial cells overexpressing Zpo2.
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