microRNA

Cell

Notch signaling pathway

Cancer

Asymmetric cell division

Signal transduction

Gene

Stem cell

Cell division

Cell biology

Carcinogenesis

Biology

Cell fate determination

Cancer stem cell

Transcription factor

Genetics

Pancreatic cancer

Cancer research

Molecular Cancer

Cancer stem cells are suggested to contribute to the extremely poor prognosis of pancreatic ductal adenocarcinoma and dysregulation of symmetric and asymmetric stem cell division may be involved. Anticancer benefits of phytochemicals like the polyphenol quercetin, present in many fruits, nuts and vegetables, could be expedited by microRNAs, which orchestrate cell-fate decisions and tissue homeostasis. The mechanisms regulating the division mode of cancer stem cells in relation to phytochemical-induced microRNAs are poorly understood.Patient-derived pancreas tissue and 3 established pancreatic cancer cell lines were examined by immunofluorescence and time-lapse microscopy, microRNA microarray analysis, bioinformatics and computational analysis, qRT-PCR, Western blot analysis, self-renewal and differentiation assays.We show that symmetric and asymmetric division occurred in patient tissues and in vitro, whereas symmetric divisions were more extensive. By microarray analysis, bioinformatics prediction and qRT-PC...

RETRACTED ARTICLE: Quercetin-induced miR-200b-3p regulates the mode of self-renewing divisions in pancreatic cancer