The utility of cellient cell blocks in low-cellularity thyroid fine needle aspiration biopsies

Published on Jun 24, 2016in Diagnostic Cytopathology1.582
· DOI :10.1002/DC.23522
Michelle Horton2
Estimated H-index: 2
Laura Been3
Estimated H-index: 3
+ 1 AuthorsS. Thomas Traweek2
Estimated H-index: 2
Background Low cellularity can be problematic in thyroid fine needle aspiration (FNA) biopsies. The Cellient cell block (CB) system has been reported to improve cell recovery compared to traditional methods. Therefore, we studied the utility of Cellient CBs in the evaluation of thyroid FNAs, with an emphasis on low-cellularity specimens. Methods ThinPrep slides were prepared from thyroid FNAs submitted in Cytolyt. After assessment using TBSRTC criteria, Cellient CBs were requested on samples with residual FNA material and an initial cytologic impression of non-diagnostic, AUS/FLUS, and on apparently benign samples with marginally adequate cellularity. The contribution of the CB findings to the final diagnosis was assessed. Results 965 cases of paired ThinPrep and CB sections were examined. Overall, the cell block findings resulted in a change of the initial ThinPrep impression in 15% (n = 148) of cases. The vast majority of these changed cases were initially inadequate for interpretation, and specifically, 31% (n = 123) of the non-diagnostic ThinPrep samples became diagnostic with a CB. The cell block findings contributed to a change in diagnosis in 8% (n = 23) of AUS/FLUS cases, and in less than 1% of low-cellularity benign samples. Conclusion The use of CBs in low-cellularity thyroid FNAs has not been well described. In this study, we found that the contribution of CBs in this setting varied by TBSRTC category. Specifically, the samples that benefited most were initially non-diagnostic specimens and select cases of AUS/FLUS, while low-cellularity benign samples gained very little additional information. Diagn. Cytopathol. 2016. © 2016 Wiley Periodicals, Inc.
📖 Papers frequently viewed together
#1S. Prendeville (Cork University Hospital)H-Index: 1
#2T. Brosnan (Cork University Hospital)H-Index: 1
Last. J. McCarthy (Cork University Hospital)H-Index: 1
view all 4 authors...
Objective Cytoblocks (CBs), or cell blocks, provide additional morphological detail and a platform for immunocytochemistry (ICC) in cytopathology. The Cellient™ system produces CBs in 45 minutes using methanol fixation, compared with traditional CBs, which require overnight formalin fixation. This study compares Cellient and traditional CB methods in terms of cellularity, morphology and immunoreactivity, evaluates the potential to add formalin fixation to the Cellient method for ICC studies and ...
#1Deepali Jain (AIIMS: All India Institute of Medical Sciences)H-Index: 26
#2Sandeep Mathur (AIIMS: All India Institute of Medical Sciences)H-Index: 16
Last. Venkateswaran K. Iyer (AIIMS: All India Institute of Medical Sciences)H-Index: 17
view all 3 authors...
The cell block (CB) is a routine procedure in cytopathology that has gained importance because of its pivotal role in diagnosis and ancillary studies. There is no precise review in the published literature that deals with the various methods of preparation of CB, its utility in diagnosis, immunocytochemistry (ICC) or molecular testing, and its drawbacks. An extensive literature search on CB in cytology using internet search engines was performed for this review employing the following keywords: ...
#1Eric Montgomery (Vassar College)H-Index: 1
#2Chen Gao (UB: University at Buffalo)H-Index: 1
Last. Lourdes Ylagan (Roswell Park Cancer Institute)H-Index: 6
view all 6 authors...
The Cellient® cell block system has become available as an alternative, partially automated method to create cell blocks in cytology. We sought to show a validation method for immunohistochemical (IHC) staining on the Cellient cell block system (CCB) in comparison with the formalin fixed paraffin embedded traditional cell block (TCB). Immunohistochemical staining was performed using 31 antibodies on 38 patient samples for a total of 326 slides. Split samples were processed using both methods by ...
#1A. M. Kruger (South Australia Pathology)H-Index: 1
#2M. W. Stevens (South Australia Pathology)H-Index: 1
Last. C. D. Carter (South Australia Pathology)H-Index: 1
view all 4 authors...
Objective To compare the CellientTM automated cell block system with the agar cell block method in terms of quantity and quality of diagnostic material and morphological, histochemical and immunocytochemical features. Materials and Methods Cell blocks were prepared from 100 effusion samples using the agar method and Cellient system, and routinely sectioned and stained for haematoxylin and eosin and periodic acid-Schiff with diastase (PASD). A preliminary immunocytochemical study was performed on...
#1John P. Crapanzano (CUMC: Columbia University Medical Center)H-Index: 9
#2Jonas J. Heymann (CUMC: Columbia University Medical Center)H-Index: 12
Last. Anjali Saqi (CUMC: Columbia University Medical Center)H-Index: 22
view all 5 authors...
Background: In the recent past, algorithms and recommendations to standardize the morphological, immunohistochemical and molecular classification of lung cancers on cytology specimens have been proposed, and several organizations have recommended cell blocks (CBs) as the preferred modality for molecular testing. Based on the literature, there are several different techniques available for CB preparation-suggesting that there is no standard. The aim of this study was to conduct a survey of CB pre...
#1Bettien M. van Hemel (UMCG: University Medical Center Groningen)H-Index: 14
#2Albert J. H. Suurmeijer (UMCG: University Medical Center Groningen)H-Index: 56
We studied the feasibility of immunocytochemistry (ICC), in situ hybridization (ISH), and polymerase chain reaction (PCR) after Cellient automated cell block processing, and tested whether methanol-based PreservCyt fixation could replace formalin fixation, in an attempt to eliminate toxic formaldehyde vapors. Immunostaining with 30 different antibodies was performed on cell blocks from 73 FNA specimens and 42 body cavity fluid specimens prepared by Cellient automated processing that uses the met...
#1Steven HechtH-Index: 1
#2Matthew McCormackH-Index: 1
Correspondence: Steven A Hecht Hologic Inc, 250 Campus Drive, Marlborough, MA 01752, USA Tel +1 508 263 8694 Fax +1 508 263 2970 Email Background: The Cellient Automated Cell Block System rapidly creates paraffin-embedded cell blocks by using vacuum filtration to deposit a layer of cells on a filter and infiltrate those cells with reagents and paraffin. This study used a “tracer” cell model to mimic low frequency abnormal cells and compare detection and representative sa...
#1Blythe GormanH-Index: 7
#2Ognjen KosaracH-Index: 5
Last. Dina R. ModyH-Index: 27
view all 5 authors...
Objective: To compare results of immunohistochemical (IHC) assays for estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor recepto
#1David G. Wagner (UNMC: University of Nebraska Medical Center)H-Index: 2
#2K C T Donna Russell (URMC: University of Rochester Medical Center)H-Index: 2
Last. F.I.A.C. Thomas A. Bonfiglio M.D. (URMC: University of Rochester Medical Center)H-Index: 2
view all 6 authors...
Traditional cell block (TCB) sections serve as an important diagnostic adjunct to cytologic smears but are also used today as a reliable preparation for immunohistochemical (IHC) studies. There are many ways to prepare a cell block and the methods continue to be revised. In this study, we compare the TCB with the Cellient™ automated cell block system. Thirty-five cell blocks were obtained from 16 benign and 19 malignant nongynecologic cytology specimens at a large university teaching hospital an...
#1Esther Diana Rossi (CUA: The Catholic University of America)H-Index: 31
#2F. Morassi (CUA: The Catholic University of America)H-Index: 1
Last. Guido Fadda (CUA: The Catholic University of America)H-Index: 44
view all 5 authors...
E. D. Rossi, F. Morassi, G. Santeusanio, G. F. Zannoni and G. Fadda Thyroid fine needle aspiration cytology processed by ThinPrep: an additional slide decreased the number of inadequate results Background: Fine needle aspiration cytology is the most accurate tool for diagnosing thyroid nodules. Its accuracy is related to the rate of inadequate samples, which can be minimized with the adoption of on-site assessment of the adequacy of the material. The introduction of liquid-based cytology (LBC) i...
Cited By8
#1Swati Satturwar (University of Pittsburgh)H-Index: 4
#2Liron Pantanowitz (UM: University of Michigan)H-Index: 59
Cell-block preparations have become an essential part of integrated cytology diagnosis. They are essentially microbiopsies that are formalin fixed and embedded in paraffin. This has become more prevalent with greater sample procurement due to the advent of newer biopsy techniques and needles. Cell-blocks allow retrieval of small tissue fragments from cytology specimens that sometimes cannot be processed by alternate cytologic techniques. They represent concentrated, cell-enriched preparations th...
#1Rana S. HodaH-Index: 1
Thyroid fine needle aspiration (FNA) is well established as a first-line diagnostic procedure in patients with thyroid nodules, but despite improved FNA techniques and increase use of ultrasound (US) guidance, about 20% of initial FNA specimens may be nondiagnostic or unsatisfactory (ND/Unsat). Nondiagnostic FNA of thyroid nodules presents a clinical dilemma, as the nature of the nodule is not clear and the nodule must be re-aspirated for a diagnosis.
#1Jesse Vance (Texas Tech University Health Sciences Center at El Paso)H-Index: 2
#2Karen Durbin (Texas Tech University Health Sciences Center at El Paso)H-Index: 1
Last. Syed M. Gilani (Texas Tech University Health Sciences Center at El Paso)H-Index: 1
view all 4 authors...
BACKGROUND: This study was conducted to evaluate the diagnostic utility of cell block material in fine needle aspiration (FNA) of thyroid nodules. DESIGN: A total of 242 thyroid fine need aspirations (FNAs) were performed between January 2015 and December 2015. Of those, all consecutive thyroid FNA cases with cell blocks (n = 140) from 129 patients (age: 58.9 ± 12.8 years) are included in this study. Cytology slides and cell blocks are reviewed for adequacy assessment based on the Bethesda Syste...
#1Vanda F. Torous (Harvard University)H-Index: 7
#2Yigu Chen (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 6
Last. Paul A. VanderLaan (BIDMC: Beth Israel Deaconess Medical Center)H-Index: 30
view all 3 authors...
Introduction Various cell block (CB) preparation methods are utilized by different laboratories, and not all laboratories perform CBs in tandem with ThinPreps (TPs). To compare the performance of different CB methods and their diagnostic value when used in conjunction with TP, we assessed the quantity and size of granulomas obtained from endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) of lymph nodes in the evaluation of granulomatous mediastinal disease. Materials an...
#1Beata Bode-Lesniewska (UZH: University of Zurich)H-Index: 17
#2B. Cochand‐Priollet (Paris V: Paris Descartes University)H-Index: 1
Last. Massimo Bongiovanni (UNIL: University of Lausanne)H-Index: 31
view all 5 authors...
: Thyroid nodules are common and are increasingly detected due to recent advances in imaging techniques. However, clinically relevant thyroid cancer is rare and the mortality from aggressive thyroid cancer remains constant. Fine needle aspiration cytology (FNAC) is a standard method for diagnosing thyroid malignancy and the discrimination of malignant nodules from goitre. As the examined nodules on thyroid FNAC are often small incidental findings, it is important to maintain a low rate of undete...
#1Pichet Sampatanukul (Chula: Chulalongkorn University)H-Index: 11
#2Andrey Bychkov (Chula: Chulalongkorn University)H-Index: 17
The assessment of adequacy is the first step in the evaluation of a thyroid fine-needle aspiration (FNA) sample. A specimen with adequate cellularity is a key to the success of thyroid cytology because it assures minimal false-negative rate. The adequacy of a thyroid FNA is defined by both the quantity and quality of the cellular and colloid components. According to the Bethesda system for reporting thyroid cytopathology, a specimen is defined as non-diagnostic if it fails to meet the adequacy c...
#1Aruna Nambirajan (AIIMS: All India Institute of Medical Sciences)H-Index: 9
#2Deepali Jain (AIIMS: All India Institute of Medical Sciences)H-Index: 26
: The cell block (CB) offers many advantages over other cytological preparations, particularly for immunocytochemical and molecular testing. However, inconsistent cellularity remains the most common reason for dissatisfaction among cytopathologists. In recent years, there has been a surge in the demand for CBs imposed by the increasing number of minimally invasive procedures performed to obtain material for diagnostic, prognostic and predictive purposes from advanced stage cancer patients. Howev...
#1Niki MargariH-Index: 14
Last. Petros KarakitsosH-Index: 34
view all 7 authors...
Objective: To investigate the potential of Classification and Regression Trees (CARTs) for the diagnosis of thyroid lesions based on cell block immunocytochemistr
This website uses cookies.
We use cookies to improve your online experience. By continuing to use our website we assume you agree to the placement of these cookies.
To learn more, you can find in our Privacy Policy.