Assessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii Sacc

Published on Jan 18, 2016in Journal of General Plant Pathology1.449
· DOI :10.1007/S10327-016-0644-0
Simi Jacob3
Estimated H-index: 3
(Jawaharlal Nehru Technological University, Hyderabad),
Ramgopal Rao Sajjalaguddam4
Estimated H-index: 4
(Sreenidhi Institute of Science and Technology)
+ 2 AuthorsHari Kishan Sudini13
Estimated H-index: 13
(ICRISAT: International Crops Research Institute for the Semi-Arid Tropics)
Sources
Abstract
Stem rot of groundnut caused by the soilborne pathogen Sclerotium rolfsii can cause significant yield losses. Biological control of stem rot using actinomycetes is a viable alternative to existing fungicidal management. Though actinomycetes are prolific antibiotic producers, reports pertaining to their use in groundnut disease management are limited. Here, actinomycetes were isolated from groundnut rhizospheric soils and screened for antagonism against S. rolfsii through a dual culture assay. Culture filtrates and crude extracts of the potential candidates were screened further for extracellular antifungal activity and characterized for biocontrol and plant-growth-promoting traits. A promising candidate was tested under greenhouse conditions as whole organism as well as crude extracts. Isolate RP1A-12 exhibited high antagonism against S. rolfsii in dual culture assay (69 % inhibition), culture filtrate assay (78–100 % inhibition at various concentrations) and crude extract assay (100 % inhibition with 1 % crude extracts). Moreover, germination of sclerotia of the test pathogen was inhibited with 1 % crude extracts. Strain RP1A-12 produced hydrogen cyanide, lipase, siderophores and indole acetic acid. Oxalic acid production by S. rolfsii was also inhibited by crude extracts of RP1A-12. In greenhouse studies, RP1A-12 reduced stem rot severity. Overall, our results suggest that isolate RP1A-12 has potential biocontrol capabilities against stem rot pathogen. Molecular characterization based on 16S rRNA gene sequencing of RP1A-12 identified it as a species of Streptomyces, closely related to S. flocculus.
Figures & Tables
Download
📖 Papers frequently viewed together
References48
Newest
#1Rahul Jog (NU: Nirma University of Science and Technology)H-Index: 7
#2Maharshi Pandya (NU: Nirma University of Science and Technology)H-Index: 5
Last. Shalini Rajkumar (NU: Nirma University of Science and Technology)H-Index: 16
view all 4 authors...
The application of plant-growth-promoting rhizobacteria (PGPR) at field scale has been hindered by an inadequate understanding of the mechanisms that enhance plant growth, rhizosphere incompetence and the inability of bacterial strains to thrive in different soil types and environmental conditions. Actinobacteria with their sporulation, nutrient cycling, root colonization, bio-control and other plant-growth-promoting activities could be potential field bio-inoculants. We report the isolation of ...
Source
#1Koichiro Tamura (Tokyo Metropolitan University)H-Index: 32
#2Glen Stecher (ASU: Arizona State University)H-Index: 8
Last. Sudhir Kumar (ASU: Arizona State University)H-Index: 83
view all 5 authors...
We announce the release of an advanced version of the Molecular Evolutionary Genetics Analysis (MEGA) software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis. In version 6.0, MEGA now enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny. A new Timetree Wizard in MEGA6 facilitates this timetree inference...
Source
#1Subramaniam Gopalakrishnan (ICRISAT: International Crops Research Institute for the Semi-Arid Tropics)H-Index: 28
#2Vadlamudi Srinivas (ICRISAT: International Crops Research Institute for the Semi-Arid Tropics)H-Index: 11
Last. Abhishek Rathore (ICRISAT: International Crops Research Institute for the Semi-Arid Tropics)H-Index: 36
view all 4 authors...
Five strains of Streptomyces (CAI-24, CAI-121, CAI-127, KAI-32 and KAI-90) were earlier reported by us as biological control agents against Fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceri (FOC). In the present study, the Streptomyces were characterized for enzymatic activities, physiological traits and further evaluated in greenhouse and field for their plant growth promotion (PGP) of sorghum and rice. All the Streptomyces produced lipase, β-1-3-glucanase and chitinase (exce...
Source
#1M. Paramasivan (TNAU: Tamil Nadu Agricultural University)H-Index: 3
#2S. MohanH-Index: 3
Last. A. Chandrasekaran (TNAU: Tamil Nadu Agricultural University)H-Index: 5
view all 4 authors...
The aim of the study for the importance of oxalic acid produced by Sclerotium rolfsii during the invasion of host tissue during pathogenesis acts synergistically with endopolygalacturonase, lowering the pH of the infected tissues to a level optimal for the activity of this enzyme. Oxalic acid was the principal toxic agent produced in the culture filtrates of S. rolfsii and it was responsible for the death of host cells. The calcium present in structural pectates can be strongly chelated by oxali...
Source
#1S. P. GawandeH-Index: 1
#2S. G. BorkarH-Index: 2
Last. Vivek P. ChimoteH-Index: 9
view all 3 authors...
Six different culture media were used to study variation in growth characteristics of ten (10) different crop isolates of S. rolfsii viz., chickpea, cotton, wheat, isabgol, groundnut, sugar beet, tuberose, mango, tomato and bottle gourd and the results indicated that the growth rate of all the isoaltes were different on different media. The mean growth rate of these isolates were in the range of 16.49 to 22.24 mm/day. On Browns agar medium some S. rolfsii isolate formed the basidial stage. The o...
#1Brahim BouizgarneH-Index: 11
The present article focuses on the role of free living bacteria in the suppression of soil-borne phytopathogens mainly Pseudomonas, Bacillus, and the actinomycetes. It also focuses on Plant –rhizobacteria interactions are involved in root colonization and molecular and biochemical basis of root colonization. Recently, the plant a ssociated bacteria called plant growth promoting rhizobacteria (PGPR) have received great attention for use as a biofertilizer and/or biopesticides for the sustainabili...
Source
Summary Antibiotics are essential for control of bacterial diseases of plants, especially fire blight of pear and apple and bacterial spot of peach. Streptomycin is used in several countries; the use of oxytetracycline, oxolinic acid and gentamicin is limited to only a few countries. Springtime antibiotic sprays suppress pathogen growth on flowers and leaf surfaces before infection; after infection, antibiotics are ineffective. Antibiotics are applied when disease risk is high, and consequently ...
Source
In principle, the isolation of secondary metabolites from microbes does not differ from their isolation from other organisms. The extraction procedure may of course be quite different, especially if it is carried out in an industrial scale, but when an extract containing the metabolites of interest is at hand, it is the same palette of adsorbents and chromatographic techniques that provide the major tools for the fractionation and eventual isolation of the pure compounds. Compared to plants, in ...
Source
#1Subramaniam Gopalakrishnan (ICRISAT: International Crops Research Institute for the Semi-Arid Tropics)H-Index: 28
#2Suresh Pande (ICRISAT: International Crops Research Institute for the Semi-Arid Tropics)H-Index: 31
Last. O P Rupela (ICRISAT: International Crops Research Institute for the Semi-Arid Tropics)H-Index: 15
view all 9 authors...
A total of 137 actinomycetes cultures, isolated from 25 different herbal vermicomposts, were characterized for their antagonistic potential against Fusarium oxysporum f. sp. ciceri (FOC) by dual-culture assay. Of the isolates, five most promising FOC antagonistic isolates (CAI-24, CAI-121, CAI-127, KAI-32 and KAI-90) were characterized for the production of siderophore, cellulase, protease, hydrocyanic acid (HCN), indole acetic acid (IAA) and antagonistic potential against Rhizoctonia bataticola...
Source
#1N.C. LêH-Index: 1
Groundnut (Arachis hypogaea L.) is an economically important legume crop in Vietnam and many other countries worldwide. S tem rot disease, caused by the soil-borne fungus Sclerotium rolfsii Sacc., is a major yield limiting factor in groundnut cultivation. Current control methods mostly rely on the extensive use of fungicides and on cultural practices. Both methods are not always effective and repeated fungicide use can lead to resistance development in the pathogen population. To further improve...
Cited By10
Newest
#1Ali WafaH-Index: 1
Source
Source
#1Kalyani Naik (KIIT: KIIT University)H-Index: 3
#2Snehasish Mishra (KIIT: KIIT University)H-Index: 15
Last. Abhishek Choudhary (KIIT: KIIT University)H-Index: 1
view all 5 authors...
Effective microbes (EM) are the coexisting naturally occurring useful microbes applied as inoculant to enhance the beneficial microflora of the soil ecosystem to facilitate agricultural production. The participating microbial consortium includes lactic acid and photosynthetic bacteria, actinomycetes, fermenting fungi, and yeast, among others. These microbes are physiologically well-matched and coexist in a provided medium. EM formulation could be applied to a target crop in the most appropriate ...
Source
#1Marika PellegriniH-Index: 10
#2Giancarlo PagnaniH-Index: 7
Last. Maddalena Del GalloH-Index: 11
view all 6 authors...
Plant growth-promoting bacteria (PGPB) afford plants several advantages (i.e., improvement of nutrient acquisition, growth, and development; induction of abiotic and biotic stress tolerance). Numerous PGPB strains have been isolated and studied over the years. However, only a few of them are available on the market, mainly due to the failed bacterial survival within the formulations and after application inside agroecosystems. PGPB strains with these challenging limitations can be used for the f...
Source
#1Syed Aripin Shariffah-Muzaimah (Malaysian Palm Oil Board)H-Index: 1
#2Abu Seman Idris (Malaysian Palm Oil Board)H-Index: 13
Last. Kamarudin Norman (Malaysian Palm Oil Board)H-Index: 1
view all 6 authors...
Abstract Basal stem rot (BSR) or Ganoderma disease is a huge problem for palm oil producers worldwide because it severely damages infected palms and significantly affects yield. Previous studies have indicated that actinomycetes have potential applications as biological control agents against a range of phytopathogens. The aim of this study was to assess whether an application of actinomycetes could potentially reduce the disease incidence in oil palm plantations. In total, 129 Streptomyces-like...
Source
#1M.M. Jurado (UAL: University of Almería)H-Index: 11
#2Francisca Suárez-Estrella (UAL: University of Almería)H-Index: 23
Last. Joaquín Moreno (UAL: University of Almería)H-Index: 24
view all 5 authors...
Abstract Strains isolated during composting processes of plant waste, and identified as Actinobacteria, proved to be significant producers of compounds that actively participate in the control of phytopathogens, such as those that cause Damping-off disease. Although most of the actinomycetes analyzed showed to be antagonistic strains against common phytopathogens, only some 30% proved to be capable of producing bioactive substances, such as siderophores, salicylic acid, chitinase enzymes or cyan...
Source
#1M. Jayaprakashvel (University of Madras)H-Index: 9
#2C. Chitra (University of Madras)H-Index: 1
Last. Narayanasamy Mathivanan (University of Madras)H-Index: 24
view all 3 authors...
Soilborne pathogenic fungi are the most serious group of plant pathogens which cause huge yield losses to crop plants. Because of the complexity in the soil environment and plant disease development, management of plant diseases caused by soilborne pathogenic fungi appears a great challenge for all times. Plant growth-promoting rhizobacteria are important soil microbial communities which are for the past few decades successfully used for the promotion of plant growth and management of plant dise...
Source
#1Simi Jacob (Jawaharlal Nehru Technological University, Hyderabad)H-Index: 3
#2Ramgopal Rao Sajjalaguddam (Jawaharlal Nehru Technological University, Hyderabad)H-Index: 1
Last. Hari Kishan Sudini (ICRISAT: International Crops Research Institute for the Semi-Arid Tropics)H-Index: 13
view all 3 authors...
Abstract Sclerotium rolfsii Sacc. is a destructive soilborne fungal pathogen with a wide host range that includes peanuts. Biological control offers an interesting alternative to fungicides for sustainable management of soilborne diseases. The current investigation is aimed at evaluating one potential biocontrol agent Streptomyces sp. RP1A-12 for growth promotion and the management of peanut stem rot disease caused by S. rolfsii under field conditions. Preliminary studies conducted under in vitr...
Source
#1Li Yulong (NWAFU: Northwest A&F University)H-Index: 2
#2Fei He (NWAFU: Northwest A&F University)H-Index: 1
Last. Quanhong Xue (NWAFU: Northwest A&F University)H-Index: 2
view all 4 authors...
Sclerotium rolfsii (Sr), a soil-borne fungal pathogen, causes disease in a wide range of crops. Recently, we identified five actinomycetes (Streptomyces globisporus subsp. globisporus, S. globisporus, S. flavotricini, S. pactum, and S. senoensis) showing significant inhibitory effects on plant pathogens. In this study, the effects of the five actinomycetes for the biocontrol of Sr were investigated using the plate culture method and microscopy examination. Two actinomycetes with higher inhibitor...
Source
#1P Kishore Varma (Acharya N. G. Ranga Agricultural University)H-Index: 1
#2S. Uppala (A&M: Texas A&M University)H-Index: 5
Last. K. Vijay Krishna Kumar (Acharya N. G. Ranga Agricultural University)H-Index: 9
view all 6 authors...
Plant-microbe interactions is an important concept, and the significance of these interactions on sustainable agriculture is enormous. These interactions can be neutral, commensal, mutualistic, saprophytic, or harmful. Endophytes are beneficial microbes that reside and establish symbiotic relationships with the plants. These beneficial microbes are of either bacterial, fungal, or actinomycete origin. A wide array of beneficial effects are reported with endophytic associations in plants that incl...
Source
This website uses cookies.
We use cookies to improve your online experience. By continuing to use our website we assume you agree to the placement of these cookies.
To learn more, you can find in our Privacy Policy.