Abstract B130: Correlation of in vitro cytotoxicity and catabolism of antibody-drug conjugates (ADCs) in target cancer cells

A sensitive and convenient ELISA method has been developed to quantify catabolites generated upon treatment of target cancer cells with an antibody-drug conjugate (ADC). The levels of catabolites generated by different ADCs in target cells were correlated with their in vitro cytotoxic potencies. The ELISA method, exemplified for maytansinoid ADCs, measures catabolite levels as low as 0.03 pmol, requiring only 1 million cells, and is suitable as...