Effect of increased billiary immunoglobulins on formation of cholesterol-phospholipid vesicle in patients with cholesterol gallstone

Objective To study the contents of Ig A, Ig G or Ig M from both bile and serum in the patients with cholesterol gallstone and control groups, and the effect of immunoglobulin on cholesterol-phospholipid vesicles. Methods Ig A, Ig G and Ig M were purified by affinity chromatography using Con A-sepharose, and(twice) Sephadex G-200 gel filtration from bile and serum. The content of Ig A, Ig G or Ig M was determined by immunodiffusion assay. The...