Isolation and Separation of Flavones from Patrinia villosa by High-Speed Counter-Current Chromatography

OBJECTIVE To develop a preparative high-speed counter-current chromatography(HSCCC) for isolating the chemical constituents from Patrinia villosa Juss.METHODS A two-phase solvent system composed of n-hexane: ethyl acetate: methanol: water(1∶(0.6∶0.6)∶1) was used.at a flow rate of 2.0 mL·min~(-1),while the apparatus rotated at 800 r·min~(-1) and the effluent was detected at 254 nm.The obtained fractions were analyzed by HPLC and identified by...