Establishment of popliteal lymph node assay model and application to evaluation of Qingkailing Injection sensitivity

OBJECTIVE To construct BALB/c mice models for direct popliteal lymph node assay(d-PLNA) and secondary popliteal lymph node assay(s-PLNA),and to investigate the sensitizing potential of Qingkailing Injection(QKLI).METHODS Female BALB/c mice were sc given D-penicillamine hydrochloride(D-Pen) 12.5,25.0,37.5,50.0 and 62.5 mg·kg-1 into the right pad of hind foot,50 μl per mouse.Mice were sacrificed on the fifth,seventh and nineth days after...