Simultaneous Determination of Vitexin and Isovitexin in Mung Bean by UPLC

The UPLC method was established for simultaneous determination of vitexin and isovitexin in mung bean.The separation was achieved on a C18 column(2.1 mm×50 mm,1.7 μm) with a mobile phase consisting of methanol-0.5% acetic acid(20:80).The flow rate was 1 mL/min.The column temperature was maintained at 30 ℃.The detection wavelength was at 338 nm.The precision,stability and repeatability of the method were good.The linear range of vitexin and...