Antibody-conjugated drug assay for protease-cleavable antibody–drug conjugates

Antibody-drug conjugates (ADCs) require multiple assays to characterize their PK. These assays can separately evaluate the ADC by quantifying the antibody or the conjugated drug and may give different answers due to assay measurement differences, heterogeneous nature of ADCs and potential biotransformations that occur in vivo.We present a new version of the antibody-conjugated drug assay for valine-citrulline-linked monomethylauristatin E...