Biology

Chromatography

Column chromatography

Noctuidae

Botany

Acetone

Larva

Phytochemical

Organic chemistry

Sesquiterpene

Spodoptera littoralis

Rutaceae

Chemistry

AUSTRALIAN JOURNAL OF BASIC AND APPLIED SCIENCES

Insecticidal and antifeedant activities of acetone extract of Casimiroa edulis (Rutaceae) leaf were screened against 4 th instar larvae of Spodoptera littoralis. Crude extract had LC50 value of 79.47μg/cm 2 and SC50 of 88.97 μg/cm 2 . Seven fractions were then collected from the extract by silica gel column chromatography (eluted by different solvent systems). Four of these fractions recorded percent sterility more than 50, in addition to, the crude extract, which gave 82.86% sterility at the LC 50 . Phytochemical analysis for crude extract and five active fractions composition were determined by GC/MS. 32 compounds were identified in the crude extract, dominated by sesquiterpene hydrocarbon, predominantly βeta caryophyllene (28.98% area), germacrene (7.73% area), in addition to sclerodione (17.63% area).Fatty acid esters with total area of 11.57% were also present. The chemical composition of the active fractions was identified. The results suggest that the acetone extract of C. edulis leaves and some of its fractions are of promising insecticidsl and antifeedant activity

Insecticidal and Antifeedant Activities and Chemical Composition of Casimiroa Edulis La Llave & Lex (Rutaceae) Leaf Extract and its Fractions Against Spodoptera Littoralis Larvae