HPLC–Tandem Mass Spectrometric Method for Rapid Quantification of Dimethylarginines in Human Plasma

Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) are derived from posttranslational methylation of arginine residues in proteins and after proteolytic release. Whereas protein methylation appears to be important in RNA binding and protein sorting, ADMA evolved as a competitive inhibitor of nitric oxide synthase at the arginine-binding site (1)(2). At a constant arginine supply, increased ADMA concentrations decrease NO...