Original paper
Receptor-Directed Chimeric Toxins Created by Sortase-Mediated Protein Fusion
Abstract
Chimeric protein toxins that act selectively on cells expressing a designated receptor may serve as investigational probes and/or antitumor agents. Here, we report use of the enzyme sortase A (SrtA) to create four chimeric toxins designed to selectively kill cells bearing the tumor marker HER2. We first expressed and purified: (i) a receptor recognition-deficient form of diphtheria toxin that lacks its receptor-binding domain and (ii) a mutated,...
Paper Details
Title
Receptor-Directed Chimeric Toxins Created by Sortase-Mediated Protein Fusion
Published Date
Aug 15, 2013
Volume
12
Issue
10
Pages
2273 - 2281