GnRH increases glucose transporter-1 expression and stimulates glucose uptake in the gonadotroph.

Published on Feb 1, 2012in Journal of Endocrinology4.041
· DOI :10.1530/JOE-11-0359
Valerie Michelle Harris1
Estimated H-index: 1
(WSU: Wayne State University),
Sachin V Bendre1
Estimated H-index: 1
(BCM: Baylor College of Medicine)
+ 5 AuthorsColleen Buggs-Saxton3
Estimated H-index: 3
(WSU: Wayne State University)
Sources
Abstract
GnRH is the main regulator of the hypothalamic–pituitary– gonadal (H–P–G) axis. GnRH stimulates the pituitary gonadotroph to synthesize and secrete gonadotrophins (LH and FSH), and this effect of GnRH is dependent on the availability of glucose and other nutrients. Little is known about whether GnRH regulates glucose metabolism in the gonadotroph. This study examined the regulation of glucose transporters (Gluts) by GnRH in the LbT2 gonadotroph cell line. Using real-time PCR analysis, the expression of Glut1, -2, -4, and -8 was detected, but Glut1 mRNA expression level was more abundant than the mRNA expression levels of Glut2, -4, and -8. After the treatment of LbT2 cells with GnRH, Glut1 mRNA expression was markedly induced, but there was no GnRH-induction of
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