Identification of Antiangiogenic and Antiinflammatory Proteins in Human Amniotic Membrane

Purpose. To identify the potential antiangiogenic and antiinflammatory proteins expressed in human amniotic membrane tissue. Methods. Human amniotic epithelial and mesenchymal cells were isolated from human amniotic membranes by sequential trypsin and collagenase digestion. Total RNAs were harvested from freshly obtained human amniotic epithelial and mesenchymal cells. Antiangiogenic and antiinflammatory proteins were detected by the reverse...