Light scattering and the absolute characterization of macromolecules

Light scattering (LS) has returned to the arsenal of analytical chemists with an impact scarcely imaginable a decade ago. Several important developments have restored this absolute measurement technique to its present status. The advent of HPSEC (high-performance size exclusion chromatography) and a variety of other techniques for macromolecular separation were of particular significance in stimulating the application of low angle laser light...