Effect of Copper on the Expression of IGF-1 from Chondrocytes in Newborn Piglets In Vitro

Published on Feb 28, 2012in Biological Trace Element Research3.738
· DOI :10.1007/S12011-012-9359-Z
Jianguo Wang12
Estimated H-index: 12
(JLU: Jilin University),
Xiaoyan Zhu8
Estimated H-index: 8
(JLU: Jilin University)
+ 8 AuthorsGuowen Liu27
Estimated H-index: 27
(JLU: Jilin University)
The experiment was performed to evaluate the influence of copper supplementation on insulin-like growth factor-1 (IGF-1) mRNA expression in chondrocytes of newborn pigs. Chondrocytes were isolated and cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in 90-mm culture plate. After 0, 12, 24, and 48 h, total RNA was isolated from chondrocytes. Then, IGF-1 mRNA expression was determined by semiquantitative RT-PCR. The results showed that the expression of IGF-1 mRNA, adjusted for β-actin expression, was increased in the culture media added to 15.6, 31.2, and 62.5 μmol/L copper, respectively. In the present experiment, the optimal copper concentration and optimal culture time for the expression of IGF-1 mRNA were 31.2 μmol/L and 48 h, respectively.
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