Pre-Steady-State Kinetic Analysis of Enzyme-Monitored Turnover during Cystathionine β-Synthase-Catalyzed H2S Generation
Abstract
Cystathionine β-synthase (CBS) catalyzes the first step in the transsulfuration pathway in mammals, i.e., the condensation of serine and homocysteine to produce cystathionine and water. Recently, we have reported a steady-state kinetic analysis of the three hydrogen sulfide (H(2)S)-generating reactions that are catalyzed by human and yeast CBS [Singh, S., et al. (2009) J. Biol. Chem. 284, 22457-22466]. In the study presented here, we report a...
Paper Details
Title
Pre-Steady-State Kinetic Analysis of Enzyme-Monitored Turnover during Cystathionine β-Synthase-Catalyzed H2S Generation
Published Date
Dec 29, 2010
Journal
Volume
50
Issue
3
Pages
419 - 425
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