Organochlorine pesticide

Catalysis

Nitrobenzene

Biology

Codonopsis

Pesticide

Chromatography

Radix (gastropod)

Heptachlor

Botany

Traditional Chinese medicine

Supercritical fluid

Alternative medicine

Extraction (chemistry)

Organic chemistry

Agronomy

Gas chromatography

Supercritical fluid extraction

Medicine

Chemistry

Pathology

Biomedical Chromatography

A method involving depuration of 12 organochlorine pesticides (OCPs) from Radix codonopsis was developed using supercritical fluid extraction (SFE). The pesticides investigated in the study included α ‐, β ‐, γ ‐ and δ ‐benzene hexachloride, PCNB (pentachloro‐nitrobenzene), PCA (pentachloroaniline), HEPT (heptachlor), MPCPS (methyl‐pentachlorophenyl sulfide), pp ′‐DDE [1,1‐dichloro‐2, 2‐bis( p ‐chlorophenyl) ethylene], op ′‐DDT [1,1,1‐trichloro‐2‐( o ‐chlorophenyl)‐2‐( p ‐chlorophenyl)ethane], pp ′‐DDD [1,1‐dichloro‐2‐2‐bis( p ‐chlorophenyl)ethane] and pp ′‐DDT [1,1,1‐trichloro‐2,2‐bis( p ‐chlorophenyl)ethane]. A series of experiments was conducted to optimize the final extraction conditions as follows: pure CO 2 , extraction pressure 15 MPa, extraction temperature 60°C, extraction time 20 min and flow rate 55 mL/h. A GC method with electron capture detection was employed for the determination of the OCPs in Radix codonopsis . An HPLC method was developed for the quantitative determination of active constituents. SFE was used to remove the organochlorine pesticide from Radix codonopsis . The results showed that at least 93.5% of the organochlorine pesticide residues in the herb sample were removed by SPE, while 95.0% of the active constituent marker (atractylenoide III) remained. Copyright © 2006 John Wiley &amp; Sons, Ltd.

Decontamination of organochlorine pesticides in <i>Radix Codonopsis</i> by supercritical fluid extractions and determination by gas chromatography

Decontamination of organochlorine pesticides in Radix Codonopsis by supercritical fluid extractions and determination by gas chromatography