Microwave-assisted, aqueous two-phase extraction was investigated to obtain effective constituents, including piceid, resveratrol and emodin in Polygonum cuspidatum. An aqueous two-phase system consisting of 25% (w/w) ethanol 21% (w/w) (NH4)2SO4 gave equal yields of piceid, and 1.1- and 1.9-times higher yields of resveratrol and emodin, respectively, than that achieved by microwave-assisted extraction and heat reflux extraction. Three-separate operations, extraction, clarification and concentration, are hereby integrated into a single step to get higher yields at lower cost. This is therefore a potentially useful method for the extraction and purification of target products.
Extraction by an aqueous two-phase system is a powerful technique for separation, concentration, and purification of biomolecules and pharmaceutical products. In the present study, the partitioning of Ciprofloxacin in aqueous two-phase system of PEG–Na2SO4–water has been investigated. The influences of temperature, salt concentration, polymer concentration and polymer molecular weight on the partitioning of Ciprofloxacin were studied. The experiments were designed using an orthogonal central com...
Last. J. Van Staden(UKZN: University of KwaZulu-Natal)H-Index: 87
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The antibacterial activity of the ethanolic root extract of Cassia occidentalis was examined. A biologically active component was isolated and identified as emodin by spectroscopic analysis. The bioactive Minimum Inhibitory Concentration (MIC) values of emodin were 7.8 × 10- 3 and 3.9 × 10- 3 mg ml- 1 against Bacillus subtilis and Staphylococcus aureus, respectively. It was not active against two Gram-negative bacteria (Klebsiella pneumoniae and Escherichia coli) at the highest concentration (5....
#1Kang Yan-fang(HEBUT: Hebei University of Technology)H-Index: 1
Two-phase aqueous system C_2H_5OH-(NH_4)2SO_4-H_2O was applied to the separation and purification of resveratrol from Polygonum cuspidatum.According to the different polarity of solvents,the different compounds of the extract were scattered in two phases.The content of resveratrol in the extract from liquid of aqueous two-phase extraction could rise up to 34.29%.Compared with the result of organic solvent extraction ,this technique has lots of advantages including less pollution,low production c...
Abstract As part of an evaluation of focused microwave-assisted extraction (FMAE) using an open-vessel system, the effects of matrix, moisture content, ageing, and solvent have been studied on the extraction efficiency of polycyclic aromatic hydrocarbons (PAHs) from spiked soils. PAHs were spiked onto three different uncontaminated air dried and originally wet soil matrices with 1- and 20-day ageing periods. Solvents used were hexane–acetone (1:1), cyclohexane–acetone (1:1) and dichloromethane. ...
Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is an active herbal component traditionally used in China for treating various ailments. Emodin exerts antiproliferative effects in many cancer cell lines and the actual molecular mechanism of which is still not clear. Since apoptosis could be a potential mechanism to explain these effects, we tested whether emodin induces cell death in human cervical cancer cells. Our results suggest that emodin exerts antiproliferative effects in human cervical c...
Abstract Tobacco has long been considered as a host to produce large quantity of high-valued recombinant proteins. However, dealing with large quantities of biomass is a challenge for downstream processing. Aqueous two-phase extraction (ATPE) has been widely used in purifying proteins from various sources. It is a protein-friendly process and can be scaled up easily. In this paper, ATPE was studied for its applicability to recombinant protein purification from tobacco with egg white lysozyme as ...
#1Tan Tian-wei(Huada: Beijing University of Chemical Technology)H-Index: 7
#2Huo Qing(Huada: Beijing University of Chemical Technology)H-Index: 1
Last. Ling Qiang(Huada: Beijing University of Chemical Technology)H-Index: 1
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Phase diagrams of alcohol (ethanol or 2-propanol)/salt (phosphate or sulfate) aqueous two-phase systems were made. The system consisting of 60% (v/v) ethanol and 15% (w/v) phosphate was then used to separate glycyrrhizin from an extract of Glycyrrhiza uralensis Fisch and gave a 92% recovery of glycyrrhizin with 2.6-fold purification.
#2Noriko Nakayama(College of Industrial Technology)H-Index: 1
Last. Satoshi Kawamura(College of Industrial Technology)H-Index: 1
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Abstract The distribution behaviour of Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and Li(I) was studied in an aqueous two-phase system formed from a polyethylene glycol and sodium sulphate in the presence of thiocyanate or iodide ion. From the distribution ratios determined as a function of the iodide or thiocyanate concentration, the extracted species were assumed. In the SCN − system Cu(II), Zn(II) and Co(II) distribute into the PEG-rich phase predominantly as Cu(SCN) 4 2− , Zn(SC...
Last. Ming-Jai Su(NTU: National Taiwan University)H-Index: 30
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Background: The major objective of the present study was to examine the cardioprotective effect of resveratrol, an antioxidant presents in red wines, in the rat after ischemia and ischemia–reperfusion (I–R). Methods : The left main coronary artery was occluded for 30 or 5 min followed by a 30-min reperfusion in anesthetized rats. Animals were preinfused with and without resveratrol before occlusion and the severity of ischemia- and I–R-induced arrhythmias and mortality were compared. Results : R...
In the present study, a microwave-assisted extraction (MAE) technique has been developed for the extraction of glycyrrhizic acid (GA) from licorice rest. Various experimental conditions, such as extraction time, different ethanol and ammonia concentration, liquid/solid ratios, pre-leaching time before MAE and material size for the MAE procedure were investigated to optimize the efficiency of the extraction. Under appropriate MAE conditions, such as extraction times of 4-5 min, ethanol concentrat...
#8Jun Wang(HBUT: Hubei University of Technology)H-Index: 13
#9Wenwen Deng(HBUT: Hubei University of Technology)H-Index: 1
Abstract The acquisition of resveratrol from Polygonum cuspidatum is complicated and costs organic solvents due to extraction and hydrolysis of its corresponding glycoside (polydatin). In this work, a novel one-pot method based on deep eutectic solvent (DES) was developed for simultaneous extraction and conversion of polydatin to resveratrol from Polygonum cuspidatum for the first time. The extraction yield of resveratrol by DES-based one-pot method were significantly higher than that of water, ...
#1Weiwei Jia(Lanzhou University of Technology)H-Index: 2
#2Zhenbin Chen(Lanzhou University of Technology)H-Index: 10
Last. Duolong Di(CAS: Chinese Academy of Sciences)H-Index: 14
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This work is focused on separation and purification of resveratrol from Polygonum cuspidatum by macroporous adsorption resin (MAR) mixed-bed technology. Firstly, the optimal MAR mixed-bed was obtai...
#1Siliang Xu(CRI: Crops Research Institute)H-Index: 1
#2Huaiyong Luo(CRI: Crops Research Institute)H-Index: 9
Last. Huifang Jiang(CRI: Crops Research Institute)H-Index: 20
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Resveratrol, a stilbene phytoalexin in plants, is believed to benefit human health. In this study, an optimized enzyme-assisted method was developed to extract the total content of trans-resveratrol (free or combined with glucose) in peanut seeds, followed by detection using high-performance liquid chromatography. The extraction process was optimized by Box-Behnken design and response surface methodology. The optimized enzyme concentration, digestion time, pH, and temperature were 3.02 g/L, 57.0...
#1Yingqin Wei(Qilu University of Technology)H-Index: 2
#2Baojuan Hou(Qilu University of Technology)H-Index: 1
Last. Feng Ma(Qilu University of Technology)H-Index: 2
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Abstract Background Salting-out extraction (SOE) had been developed as a special branch of aqueous two-phase system recently. So far as we know, few reports involved in extracting ginsenosides with SOE because of the lower recovery caused by the unique solubility and surface activity of ginsenosides. A new SOE method for rapid pretreatment of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium was established in this article. Methods The SOE system comprising ethanol and sodium c...
#1Liaqat Zeb(DUT: Dalian University of Technology)H-Index: 1
#2Xudong Wang(DUT: Dalian University of Technology)H-Index: 6
Last. Zhilong Xiu(DUT: Dalian University of Technology)H-Index: 45
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Abstract The extraction and separation of docosahexaenoic acid (DHA)-rich oils, proteins, and polysaccharides from microalgae is a challenge for industrial commercialization of DHA, such as drying and dewatering, cell wall disruption, laborious conventional extraction procedures and exploitation of large volumes of solvents. To address these concerns, a novel microwave-assisted three-liquid-phase salting-out extraction (MA-TLPSOE) was used to separate DHA-rich oils, proteins, and polysaccharides...
#1Yuesheng Dong(DUT: Dalian University of Technology)H-Index: 11
#2Fan Yang(DUT: Dalian University of Technology)H-Index: 66
Last. Zhilong Xiu(DUT: Dalian University of Technology)H-Index: 45
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Abstract Separation and purification of secondary metabolites from wild microorganisms remains a challenge due to the analogues existing in the fermentation broth. In this study, a three-liquid-phase salting-out extraction system (TLP-SOES) coupled with column chromatography was developed to purify wortmannilactone F and its dehydrated derivative wortmannilactone L. The TLP-SOES composed of 25.5% petroleum ether/20.0% ethanol/12.9% ammonium sulfate (w/w) at pH 5.6 and room temperature was found ...
Abstract An efficient ultrasound-assisted aqueous two-phase extraction (UAATPE) and enrichment process for resveratrol from the enzymatic hydrolysates of Polygonum cuspidatum using an ethanol/ammonium sulfate system was developed. After enzymatic hydrolysis using polydatin-β-glucosidase (PBG) which was a wild extracellular enzyme, 99.7% of polydatin was converted to resveratrol. The maximum yield of resveratrol was 10.7 mg/g and the corresponding recovery rate was 99.1% at 21% (w/w) ethanol/22% ...
Last. Hanqi Zhang(JLU: Jilin University)H-Index: 59
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Abstract Ionic liquid-based salt-induced liquid-liquid extraction was developed for the first time and applied to the extraction of four active constituents, including polydatin, resveratrol, emodin, and physcion in Polygonum cuspidatum (P. cuspidatum). In this study, ionic liquid was used as extraction solvent. The dried P. cuspidatum samples purchased from the pharmacy were triturated and passed through a 120-mesh sieve. The obtained sample powders were dried to constant weight at 55 ℃, and th...
Last. James Z. Tang(University of Wolverhampton)H-Index: 14
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Abstract A rapid method for simultaneous extraction and separation of multiple alkaloids from Radix Sophorae Tonkinensis (RST) was developed by microwave-assisted aqueous two-phase extraction (MAATPE) using the aqueous two-phase extraction system (ATPS) of ethanol/Na2HPO4 as the extraction solvent. The effects of key factors on extraction yield were investigated by utilizing single-factor experiment coupled to response surface methodology (RSM). The regression model by RSM was significant (p
