Rapid modulatory effect of estradiol on acetylcholine-induced Ca²⁺signal is mediated through cyclic-GMP cascade in LHRH-releasing GT1-7 cells

Hypothalamic luteinizing hormone‐releasing hormone neurons (LHRH) form the final pathway for the central control of reproduction through the release of LHRH into the pituitary‐hypothalamic system. We previously found that LHRH‐producing GT1‐7 cells respond to acetylcholine (ACh) with an increase in intracellular calcium ([Ca 2+ ] i ) through activation of muscarinic receptors. This effect is acutely modulated by 17β‐estradiol in a manner...