Streptozocin

Streptozotocin

Cytochrome c

Calcium

Endocrinology

Glycation

Biochemistry

Glycosylation

Organic chemistry

Cytochrome

Enzyme

Internal medicine

Advanced glycation end-product

Mitochondrion

Medicine

Chemistry

Diabetes mellitus

The FASEB Journal

The present study was undertaken to observe the changes of mitochondrial calcium and cytochrome C in noninsulin dependent diabetes mellitus (NIDDM) rats. Methods and Results: Fifteen male NIDDM rats were made by injection of 2% streptozocin (25mg/kg, i.v.) and fed with high fat diet for 10 weeks. 10 male normal rats were used as control. At week 10, didymus tissue was harvested and separated, and mitochondrial calcium (by flame atomic absorption method), cytochrome C (by Zhang’s method) and end product of glycosylation (by fluorospectrophotometry) were measured. The results showed that mitochondrial calcium (2.38±0.36 vs 2.79±0.18 mg/L) and cytochrome C (1.79±0.34 vs 2.17±0.23 mmol/L) were lower (NIDDM rats vs control rats, P<0.05) in didymus tissues from NIDDM rats compared with control. Cytoplasm cytochrome C (2.40±0.39 vs 1.54±0.48 mmol/L) and end product of glycosylation (17.01±1.80 vs 12.90±1.36) were higher (NIDDM rats vs control rats, P<0.05) in didymus tissues from NIDDM rats compared with control. Conclusions: The decreases in mitochondrial calcium and cytochrome C, and increase in end product of glycosylation may be adverse factors to didymus tissues of diabetic rats.

Changes of mitochondrial calcium and cytochrome C in didymus tissues from diabetic rats