Abstract Colostrinin™ (CLN), a proline-rich polypeptide complex, originally isolated from ovine colostrum, has been proven to be a promising and non-toxic natural preparation capable of preventing the development of Alzheimer's disease (AD). Utilizing a new purification protocol, colostrum from cows, goats or humans can also be used as alternative sources of this complex. Comparison of properties of preparation derived from these three species revealed no significant differences from that obtained from sheep. In amino-acid composition, as in ovine CLN, a high level of proline residues (over 20%) and acidic amino acids (approximately 18%) was found in CLN from cows, goats and humans. The comparison of the electrophoretic patterns and reversed-phase-high performance liquid chromatography (RP-HPLC) elution profiles showed the presence of additional proteins in the CLN preparations of different origin, consisting mainly of β-lactoglobulin and α-lactalbumin. These proteins could be removed by an ultrafiltration process. Colostrum, regardless of origin (ovine, bovine, caprine or human), appeared to be a rich source of CLN fractions that could be separated in gram quantities. Storage of colostrum in frozen or lyophilized form affected neither the quantity nor quality of the purified peptides compared with its preparation from fresh material.
#1Bo Su(Case Western Reserve University)H-Index: 21
#2Xinglong Wang(Case Western Reserve University)H-Index: 45
Last. Xiongwei Zhu(Case Western Reserve University)H-Index: 105
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Multiple lines of evidence demonstrate that oxidative stress is an early event in Alzheimer’s disease (AD), occurring prior to cytopathology, and therefore may play a key pathogenic role in AD. Oxidative stress not only temporally precedes the pathological lesions of the disease but also activates cell signaling pathways, which, in turn, contribute to lesion formation and, at the same time, provoke cellular responses such as compensatory upregulation of antioxidant enzymes found in vulnerable ne...
#2Maria Janusz(PAN: Polish Academy of Sciences)H-Index: 16
Last. Józef Lisowski(PAN: Polish Academy of Sciences)H-Index: 16
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Abstract A proline-rich polypeptide complex (PRP) has immunoregulatory properties and also shows beneficial effects in Alzheimer's disease (AD). It is known that the unregulated activation of microglial cells in AD may result in chronic inflammatory response. There is a link between the activation of immune cells on the periphery and in the central nervous system (CNS). Therefore, we studied the effect of the PRP on human peripheral blood mononuclear cells (PBMCs) stimulated by LPS with PHA (LP)...
Last. Istvan Boldogh(UTMB: University of Texas Medical Branch)H-Index: 64
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Abstract Colostrinin™ (CLN), a uniform mixture of low-molecular weight, proline-rich polypeptides, induces neurite outgrowth of pheochromocytoma cells and inhibits beta amyloid-induced apoptosis. Moreover, its administration to patients with Alzheimer's disease resulted in improved cognitive functions. In this study, we investigated the impact of CLN on the lifespan of murine diploid fibroblast cells (MDF), an in vitro model for cellular aging. Here, we show that CLN significantly decelerates th...
Abstract Oxidative damage is a major feature in the pathophysiology of Alzheimer's disease (AD). In this review, we discuss free radical-mediated damage to the biochemical components involved in the pathology and clinical symptoms of AD. We explain how amyloid beta-protein (Aβ), microtubule-associated protein tau, presenilins, apolipoprotein E, mitochondria and proteases play a role in increasing oxidative stress in AD. Aβ not only can induce oxidative stress, but its generation is also increase...
Abstract Colostrinin (CLN) is a biologically active proline-rich polypeptide which has therapeutic potential for the alleviation of memory deficits in age-related dementias in a number of human conditions, particularly Alzheimer’s disease. To examine the efficacy of CLN in other species, day-old domestic chicks were used as a model system to study its effects on retention of memory for a single one-trial learning paradigm—avoidance of a bitter-tasting substance (methylanthranilate, MeA). Birds w...
Last. Józef Lisowski(PAN: Polish Academy of Sciences)H-Index: 16
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Abstract A proline-rich polypeptide complex (PRP) with immunoregulatory and procognitive activities shows beneficial effects in Alzheimer's disease (AD). The mechanism of action of PRP in AD is not yet clarified. Here, we present results of the effect of PRP on Vitamin D 3 -induced phenotypic (CD11b and CD14) and functional (phagocytic) differentiation/maturation of monocytes/macrophages using the premonocytic HL-60 cell line as a model. This cell line can be induced to differentiate into monocy...
Abstract Colostrinin (CLN), a mixture of proline-rich polypeptides, has shown a stabilizing effect on cognitive function in Alzheimer’s patients measured by the Alzheimer’s disease Assessment Scale-cognitive (ADAS-cog) and in Instrumental Activities of Daily Living (ILDL) in recently conducted clinical trials. The aim of this study was to elucidate a possible mode of action of CLN in the treatment of Alzheimer’s disease. Here, we report that CLN prevents the aggregation of β-amyloid peptide Aβ (...
#2Maria Janusz(PAN: Polish Academy of Sciences)H-Index: 16
Last. Józef Lisowski(PAN: Polish Academy of Sciences)H-Index: 16
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Abstract A proline-rich polypeptide complex (PRP) isolated from ovine colostrum shows immunoregulatory and procognitive activities. It shows beneficial effects in Alzheimer's disease (AD) patients when orally administered in the form of tablets called Colostrinin®. The mechanism of action of PRP/Colostrinin® in AD has not been yet clarified. It is known that oxidative stress and overproduction of NO may enhance neurodegenerative processes. PRP regulates the secretion of cytokines, inhibits NO an...
#1Xiongwei Zhu(Case Western Reserve University)H-Index: 105
#2Arun K. Raina(Case Western Reserve University)H-Index: 36
Last. George Perry(Case Western Reserve University)H-Index: 162
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Multiple lines of evidence demonstrate that oxidative stress is an early event in Alzheimer's disease (AD), occurring prior to cytopathology, and therefore may play a key pathogenic role in the disease. Indeed, that oxidative mechanisms are involved in the cell loss and other neuropathology associated with AD is evidenced by the large number of metabolic signs of oxidative stress as well as by markers of oxidative damage. However, what is intriguing is that oxidative damage decreases with diseas...
#2Łukasz Bobak(Wroclaw University of Environmental and Life Sciences)H-Index: 8
Last. Aleksandra Zambrowicz(Wroclaw University of Environmental and Life Sciences)H-Index: 10
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The results of our research have proven that yolkin preparations isolated from eggs of different bird species show a high similarity in polypeptide composition. Despite the small differences in protein patterns, all of yolkin preparations showed also strong immunomodulatory activity, comparable with yolkin obtained previously from hen egg yolk. It can therefore be deducted that the presence of this polypeptide complex in the egg is not accidental and performs an important biological function for...
Last. Jakub Fichna(Medical University of Łódź)H-Index: 35
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Inflammatory bowel disease (IBD) is a group of chronic relapsing disorders whose etiology has not been fully explained. Therefore, available therapeutic approaches for IBD patients are still insufficient. Current treatment strategies are targeted to immune system dysfunctions, often associated with alternations in the microbiota, which contribute to the development of chronic intestinal inflammation. Therapeutics include anti-inflammatory drugs such as aminosalicylates and corticosteroids, immun...
Colostral proline-rich polypeptide complex (PRP) with immunoregulatory and procognitive activities shows beneficial effects in Alzheimer’s disease (AD). As the laboratory method of isolating PRP is expensive, laborious, and time consuming, a new large-scale methanol method of PRP isolation was developed. The proline-rich polypeptide complex obtained by this new method (named methanol PRP–MPRP) from both ovine or bovine colostra shows psychotropic activity and inhibitory effect on amyloid β aggre...
#1Sanket Borad(NDRI: National Dairy Research Institute)H-Index: 7
#2Ashish Kumar Singh(NDRI: National Dairy Research Institute)H-Index: 20
Abstract Colostrum contains an abundance of immune components and can be exploited to address the poor immune status of developing countries' populations. The ability of colostrum immunoglobulins (Igs) to offer passive immunity to calves has been validated previously; the equivalent of only 7–10% of a calf's bodyweight of colostrum is required for passive immunisation. To transfer the immunotherapeutic properties of colostrum to the human being, the excess colostrum left over after feeding calve...
Colostrum and milk are the initial mammalian nourishment and rich reservoir of essential nutrients for newborn development. Bioactive peptides isolated from natural sources, such as colostrum, serve as endogenous regulators and can be used as alternative therapeutic agents in the treatment of neurodegenerative diseases. One example is the previously unknown NP-POL nonapeptide isolated from Colostrinin. In the present study, we investigated a method of NP-POL nonapeptide isolation using Bio-Gel P...
Last. Tadeusz Trziszka(Wroclaw University of Environmental and Life Sciences)H-Index: 16
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Abstract A large number of bioactive peptides isolated from natural sources are known to play important physiological roles in the human body. It is possible to use these as alternative therapy agents. One example is yolkin which can be useful as a food supplement, a natural therapeutic agent for preventing and treating cognitive disorders of various origins, preferably in patients with unsatisfactory responses to known therapies. A new simple method of isolation of yolkin based on precipitation...
: This paper reviews the composition of colostrum and the potential preventive and therapeutic use of this "first milk" for treating various gastrointestinal disorders in humans. Colostrum is a complex biological liquid that is richer in antimicrobial peptides, immune-regulating compounds and growth factors than the subsequent mature milk. The main functions of colostrum are to provide essential nutritional components, strengthen the natural defense system, modulate immune response, balance inte...
The study aimed to assess the effect of the polypeptide Y complex (Yolkin), isolated from chicken egg yolk, on behavioural and cognitive functions. It also aimed to compare this activity with colostrum-derived substances (Colostrinin, Coloco), which have a confirmed impact on learning and memory. In the study, the effect of Yolkin, administered to rats of different ages, who performed various tasks involving spatial and episodic memory, motor functions and exploratory behavior, was assessed. The...
#2Agnieszka Sosnowska(Wroclaw University of Environmental and Life Sciences)H-Index: 3
Last. Antoni Polanowski(Wroclaw University of Environmental and Life Sciences)H-Index: 7
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In the hen immune system the egg content plays as significant a role in the development of the chick as colostrum does in newborn mammals. One of the most important proteins in this system seems to be the main yolk immunoglobulin IgY. It has been shown that IgY is accompanied by an immunostimulatory polypeptide complex named yolkin. In this report the biological activities of yolkin separated by means of four different procedures are presented. It was shown that yolkin acts as an inducer rather ...