Burn scar lipofilling: immunohistochemical and clinical outcomes

Published on Sep 1, 2013in Journal of Craniofacial Surgery0.953
· DOI :10.1097/SCS.0B013E3182A148B9
Agostino Bruno2
Estimated H-index: 2
Giancarlo delli Santi2
Estimated H-index: 2
+ 3 AuthorsPaolo Palombo5
Estimated H-index: 5
Abstract Over the past years, lipofilling has been used for many pathologies and sequelae; recently, its use has also widened for burn outcomes. A total of 93 burn scars were assessed (from September 2011 to February 2012). Half of scar area was treated through injection of adipose tissue harvested from subcutaneous fat and processed in accordance with the Coleman technique. Biopsy specimens were taken before treatment and at 3 and 6 months after the treatment. Histologic and immunohistochemical evaluations were conducted. In the samples examined, a marked improvement was observed, which can be seen in 3 months, but most of all, documented at 6 months. In addition, from a clinical point of view, an improvement has been documented, both functional and aesthetic. Lipofilling for burn scar complete and improve the results of standard surgical procedures, with long-term care setting and long-acting results.
📖 Papers frequently viewed together
126 Citations
288 Citations
40 Citations
#1Chenyu Huang (Nippon Medical School)H-Index: 21
#2Satoshi Akaishi (Nippon Medical School)H-Index: 25
Last. Rei Ogawa (Nippon Medical School)H-Index: 10
view all 3 authors...
Mechanotransduction is the process by which physical forces are sensed and converted into biochemical signals that then result in cellular responses. The discovery and development of various molecular pathways involved in this process have revolutionized the fundamental and clinical understanding regarding the formation and progression of cutaneous scars. The aim of this review is to report the recent advances in scar mechanosignaling research. The mechanosignaling pathways that participate in t...
53 CitationsSource
21 CitationsSource
#1Hana Farhangkhoee (Sunnybrook Health Sciences Centre)H-Index: 1
#2Karen M. Cross (Sunnybrook Health Sciences Centre)H-Index: 7
Last. Joel S. Fish (Sunnybrook Health Sciences Centre)H-Index: 4
view all 5 authors...
: Histological diagnosis of burn depth lacks consensus. The purpose of this study was to determine whether Ki-67, a cell proliferation marker, provides an index of integument viability after burn injury. Induction of thermal burn injuries (3, 12, 20, 30, 75, 90, and 120 seconds) were made with a brass rod heated to 100°C on the dorsal trunk of the swine. Controls were created with a brass rod heated to 37.5°C. Four 6-mm biopsies were obtained from each site for histological analysis of Ki-67. Bi...
6 CitationsSource
#1Patrick H. Blit (Sunnybrook Research Institute)H-Index: 11
#2Marc G. Jeschke (Sunnybrook Research Institute)H-Index: 11
9 CitationsSource
#1Ayako Ito (Tottori University)H-Index: 4
#2Nanako Yamada (Tottori University)H-Index: 11
Last. Osamu Yamamoto (Tottori University)H-Index: 16
view all 5 authors...
Herein, we report the investigation of two cases of atypical fibroxanthoma (AFX). One AFX developed within actinically damaged skin, as is typical, while the other developed within a burn scar within non-sun-exposed skin. The two tumors showed almost identical histopathological, immunohistochemical and ultrastructural features. The tumors were composed of pleomorphic spindled, epithelioid, multinucleated and bizarre cells with enlarged atypical nuclei. Most tumor cells expressed vimentin and abo...
12 CitationsSource
#1Vincent Flacher (Innsbruck Medical University)H-Index: 16
#2Christoph H. Tripp (Innsbruck Medical University)H-Index: 21
Last. Nikolaus Romani (Innsbruck Medical University)H-Index: 75
view all 11 authors...
Antigen-presenting cells can capture antigens that are deposited in the skin, including vaccines given subcutaneously. These include different dendritic cells (DCs) such as epidermal Langerhans cells (LCs), dermal DCs, and dermal langerin + DCs. To evaluate access of dermal antigens to skin DCs, we used mAb to two C-type lectin endocytic receptors, DEC-205/CD205 and langerin/CD207. When applied to murine and human skin explant cultures, these mAbs were efficiently taken up by epidermal LCs. In a...
114 CitationsSource
#1Marco KlingerH-Index: 20
#2Mario MarazziH-Index: 21
Last. Maria Luisa Torre (UNIPV: University of Pavia)H-Index: 28
view all 4 authors...
Background Despite civilization and progress, burns occur frequently in the world. Remarkable discoveries of wound healing mechanisms have been reported. On the other hand, long-term outcomes from burn injuries represent a barrier to improvement of patients’ social, functional, and psychological condition. Lipofilling, described since the 1980s, currently is used for several clinical applications. This study aimed to verify whether lipofilling could ameliorate scar remodeling in three clinical c...
288 CitationsSource
Abstract At present, various scar assessment scales are available, but not one has been shown to be reliable, consistent, feasible, and valid at the same time. Furthermore, the existing scar assessment scales appear to attach little weight to the opinion of the patient. The newly developed Patient and Observer Scar Assessment Scale consists of two numeric scales: the Patient Scar Assessment Scale (patient scale) and the Observer Scar Assessment Scale (observer scale). The patient and observer sc...
616 CitationsSource
Keloids and hypertrophic scars (HSc) are unique human dermal fibroproliferative disorders (FPD) that occur following trauma, inflammation, surgery, and burns and possibly spontaneously. Keloids occur in individuals with a familial predisposition, enlarge and extend beyond the margins of the origin wounds, and rarely regress (Fig. 1). HSc are raised, erythematous, pruritic, fibrous lesions that typically remain within the confines of the original wound, usually undergo at least partial spontaneou...
298 CitationsSource
The development of scars in 121 burned children, 1-16 years old, was studied by examining 252 biopsies of granulation tissue. The range in age of the granulation tissue subsequent to the initial injury was from 20 to more than 120 days. The follow-up data of the patients spanned a period from 2 months to 4 years after burn. One hundred of the patients developed hypertrophic scars and their granulation tissue showed collagen fibers in a whorl-like or nodular arrangement. This was in contrast to t...
38 CitationsSource
Cited By53
#1Qiang Dai (Peking Union Medical College)
#2Qian Yu (Peking Union Medical College)
Last. Lei Cai (Peking Union Medical College)
view all 4 authors...
BACKGROUND Once an early-stage immature scar develops into a hypertrophic scar, it is difficult to achieve satisfactory improvement with existing treatments. Therefore, the prevention of hypertrophic scar formation should be given priority. Fat grafting was previously proven to be an effective treatment for mature scars, but it has not been used in the early-stages of (less than three months) scar formation. OBJECTIVES We present our experience with the use of combined grafting of macro fat and ...
Abstract Introduction Microfat grafting is a well-known technique that is underutilized in dermatology. Instead of removing sclerotic tissue, microfat grafting preserves the tissue and uses stem cells for remodeling its structure into normal tissue. We performed a retrospective study of patients treated with microfat grafting for sclerotic and atrophic skin lesions and scars. Patients and methods Seventy-two microfat grafts were performed using the Magalon technique under general anaesthesia for...
#1Jérôme Laloze (University of Limoges)H-Index: 6
#2Loïc Fievet (University of Toulouse)H-Index: 1
Last. Alexis Desmoulière (University of Limoges)H-Index: 49
view all 3 authors...
Significance: Wound healing is a complex process involving pain and inflammation, where innervation plays a central role. Managing wound healing and pain remains an important issue especiall...
1 CitationsSource
#1Mervi Laukka (UTU: University of Turku)H-Index: 1
#2Erika Hoppela (TYKS: Turku University Hospital)H-Index: 3
Last. Pauliina Hartiala (UTU: University of Turku)H-Index: 11
view all 14 authors...
Background Adhesion formation contributes to postoperative complications in abdominal and gynaecological surgery. Thus far, the prevention and treatment strategies have focused on mechanical barriers in solid and liquid form, but these methods are not in routine use. As autologous fat grafting has become popular in treatment of hypertrophic scars because of its immunomodulatory effects, we postulated that fat grafting could also prevent peritoneal adhesion through similar mechanisms.
#1Todor K. Krastev (Maastricht University Medical Centre)H-Index: 7
#2S. J. Schop (Maastricht University Medical Centre)H-Index: 2
Last. René R. W. J. van der Hulst (Maastricht University Medical Centre)H-Index: 27
view all 5 authors...
SUMMARY Background Autologous fat transfer (AFT) also known as lipofilling, has been demonstrated to be more than just a filler. Through both mechanical dissection and local tissue remodeling mediated by stem cells, it is thought to improve scar quality, function and even pain. This paper aims to investigate the evidence regarding its safety and effectiveness for treating fibrosis and scar-related conditions. Methods A literature search was performed in PubMed, Embase and the Cochrane Library to...
1 CitationsSource
#1Mimi R. Borrelli (Stanford University)H-Index: 13
#2Ronak A. Patel (Stanford University)H-Index: 4
Last. Derrick C. Wan (Stanford University)H-Index: 37
view all 14 authors...
Fat grafting can reduce radiation-induced fibrosis. Improved outcomes are found when fat grafts are enriched with adipose-derived stromal cells (ASCs), implicating ASCs as key drivers of soft tissue regeneration. We have identified a subpopulation of ASCs positive for CD74 with enhanced antifibrotic effects. Compared to CD74- and unsorted (US) ASCs, CD74+ ASCs have increased expression of hepatocyte growth factor, fibroblast growth factor 2, and transforming growth factor β3 (TGF-β3) and decreas...
Resume Introduction La prise en charge chirurgicale des dermo-hypodermites bacteriennes necrosantes et fasciites necrosantes (DHBN-FN) des membres entraine des pertes de substance importantes. Cette etude consiste a mesurer l’impact fonctionnel et la qualite de vie chez les survivants. Patients et methodes Nous rapportons une etude bicentrique retrospective de 62 patients pris en charge pour une DHBN-FN des membres entre 2000 et 2017. Les donnees demographiques, cliniques, biologiques et chirurg...
BACKGROUND: Autologous fat graft is often employed to treat body contour defects. There is currently increased interest in the regenerative properties of fat grafting. OBJECTIVES: The authors evaluated the histological changes of fat grafting in a blinded randomized controlled trial of staged fat grafting-abdominoplasty. METHODS: Ten women between 24 and 55 years of age with a body mass index <30 kg/m2 and previous cesarean scar were submitted to fat grafting followed by staged abdominoplasty. T...
3 CitationsSource
#1Mimi R. Borrelli (Stanford University)H-Index: 13
#2Nestor M. Diaz Deleon (Stanford University)H-Index: 2
Last. Derrick C. Wan (Stanford University)H-Index: 37
view all 11 authors...
The aim of this study was to explore the therapeutic effects of fat grafting on radiation-induced hind limb contracture. Radiation therapy (RT) is used to palliate and/or cure a range of malignancies but causes inevitable and progressive fibrosis of surrounding soft tissue. Pathological fibrosis may lead to painful contractures which limit movement and negatively impact quality of life. Fat grafting is able to reduce and/or reverse radiation-induced soft tissue fibrosis. We explored whether fat ...
5 CitationsSource