Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
Abstract
Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic...
Paper Details
Title
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
Published Date
Mar 2, 2014
Journal
Volume
11
Issue
4
Pages
410 - 412
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