Hydrodynamic chromatography: packed columns, multiple detectors, and microcapillaries

Published on Jan 1, 2012in Analytical and Bioanalytical Chemistry4.157
· DOI :10.1007/S00216-011-5334-3
André M. Striegel25
Estimated H-index: 25
(FSU: Florida State University)
Sources
Abstract
Hydrodynamic chromatography (HDC) is a liquid chromatographic technique that separates analytes on the basis of their size in solution. Separation can be conducted either in an open tube or in a column packed with inert, nonporous beads. In HDC, larger analytes elute first and smaller ones later, due to preferential sampling of the streamlines of flow in the open tube or in the interstitial medium of the packed column. Because of the low shear rates experienced in HDC, coupled with the wealth of information obtained when employing a multiplicity of detection methods, the technique has experienced a resurgence in recent years in both the particle sizing and macromolecular arenas, where it can provide information on the mutual interdependence of molar mass, size, shape, and compactness. Additionally, microcapillary HDC is also gaining popularity amongst the bioanalytical community, who have employed the technique, inter alia, to separate DNA fragments over a base pair range spanning four orders in magnitude. Here, examples from the literature are used to show how HDC has been applied in each of the aforementioned areas, explaining the information that can be obtained from various detector combinations, and opining on the future of the technique.
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References31
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#1Kelvin J. Liu (Johns Hopkins University)H-Index: 15
#2Tushar D. RaneH-Index: 11
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Single-molecule free solution hydrodynamic separation (SML-FSHS) cohesively integrates cylindrical illumination confocal spectroscopy with free solution hydrodynamic separation. This technique enables single-molecule analysis of size separated DNA with 100% mass detection efficiency, high sizing resolution and wide dynamic range, surpassing the performance of single molecule capillary electrophoresis. Furthermore, SML-FSHS required only a bare fused silica microcapillary and simple pressure cont...
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#1Amandaa K. Brewer (FSU: Florida State University)H-Index: 6
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The string-of-pearls-type morphology is ubiquitous, manifesting itself variously in proteins, vesicles, bacteria, synthetic polymers, and biopolymers. Characterizing the size and shape of analytes with such morphology, however, presents a challenge, due chiefly to the ease with which the “strings” can be broken during chromatographic analysis or to the paucity of information obtained from the benchmark microscopy and off-line light scattering methods. Here, we address this challenge with multide...
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#1Imad A. Haidar Ahmad (FSU: Florida State University)H-Index: 12
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Sequence length heterogeneity (SLH) is defined as the change, as a function of copolymer molar mass (M), in the average number of continuous monomers of a given repeat unit. SLH can influence polymeric properties such as thermal stability, mechanical behavior, transparency, and the ability of copolymers to reduce interfacial surface tension. Here, we demonstrate the relation between SLH and the change as a function of molar mass of a dimensionless size parameter, the ratio of the viscometric rad...
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Starch and glycogen are highly branched polymers of glucose of great importance to humans in managing and mitigating nutrition-related diseases, especially diabetes and obesity, and in industrial uses, for example in food and paper-making. Size-separation characterization using multiple-detection size-exclusion chromatography (SEC, also known as gel-permeation chromatography, GPC) is able to furnish substantial amounts of information on the relationships between the biosynthesis, processing, str...
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#1Agnès Rolland-Sabaté (INRA: Institut national de la recherche agronomique)H-Index: 22
#2Sophie Guilois (INRA: Institut national de la recherche agronomique)H-Index: 12
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Starch consists of a mixture of two α-glucans built mainly upon α-(1,4) linkages: amylose, an essentially linear polymer, and amylopectin, a branched polymer containing 5–6% of α-(1,6) linkages. The aim of the present work was to analyze the structural properties of native starches displaying different amylose-to-amylopectin ratios and arising from different botanical sources, using asymmetrical flow field flow fractionation (A4F) and a combination of hydrodynamic and size-exclusion chromatograp...
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#1Amandaa K. Brewer (FSU: Florida State University)H-Index: 6
#2André M. Striegel (FSU: Florida State University)H-Index: 25
The biological application of nanoparticles has resulted in an increased need for the development of robust, accurate, and precise methods for quality control analysis and characterization. Parameters such as particle size, particle shape, and their distributions affect end-use properties such as chemical reactivity, diffusivity, permeability, and transport. Introduced here is a hydrodynamic chromatography (HDC) method utilizing multi-angle static light scattering, quasi-elastic light scattering...
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#1Amandaa K. Brewer (FSU: Florida State University)H-Index: 6
#2André M. Striegel (FSU: Florida State University)H-Index: 25
A detailed quantitative description of particle size, shape, and their distributions is essential for understanding and optimization of the solid-, solution-, and melt-state properties of materials. Here, we employ quadruple-detector hydrodynamic chromatography (HDC) with multi-angle static light scattering, quasi-elastic light scattering, differential viscometry, and differential refractometry detection as a method for characterizing three important physical properties of materials, namely the ...
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#1Amandaa K. Brewer (FSU: Florida State University)H-Index: 6
#2André M. Striegel (FSU: Florida State University)H-Index: 25
The ability to characterize the size and shape distributions of broadly polydisperse analytes is a driving force in particle size analysis. Multi-detector hydrodynamic chromatography (HDC), which has previously shown promise in its ability to characterize the size and shape of monodisperse, spherical polystyrene latex standards, is applied here to include the characterization of bi-, tri-, and tetramodal latex blends and their constituents varying in size, chemistry, and compactness. The ability...
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#1Samantha L. Isenberg (FSU: Florida State University)H-Index: 2
#2Amandaa K. BrewerH-Index: 6
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Alternan is an ultrahigh molar mass polysaccharide composed of alternating α-(1→3) and α-(1→6) repeat units and that also possesses long-chain branching. Its molar mass distribution (MMD) can extend into the hundreds of millions of grams per mole. Characterizing alternan by means of size exclusion chromatography (SEC) is a lengthy process and an incomplete one because even under the best possible experimental conditions, the polysaccharide appears to degrade during its passage through the SEC co...
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Abstract null null In a recent article [Analytical Chemistry, 93(17), 6808-6816 (2021)], an unconventional device configuration enforcing a Brownian sieving mechanism was proposed as proof of concept for the efficient implementation of microcapillary hydrodynamic chromatography (MHDC). In this article, we perform a thorough analysis of the device geometry and of operating conditions, in order to single out the optimal configuration maximizing separation resolution. Brenner’s macro-transport theo...
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Hydrodynamic chromatography (HDC) is a size-based liquid chromatographic technique with similar applications as size exclusion chromatography (SEC) and flow field flow fractionation (flow FFF). Separation in HDC is induced within the parabolic flow velocity profile in an open tube or in the interstitial spaces of a packed column, whereby analytes preferentially sample the various streamlines of flow based on size and as a result the larger analytes elute prior to the smaller ones. HDC experiment...
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