Gene

Linguistics

Philosophy

Molecular biology

Biology

Transcription (linguistics)

Bacteriophage

Strain (injury)

Genetics

Anatomy

Lambda phage

Escherichia coli

T7 RNA polymerase

RNA polymerase

Gene expression

An Escherichia coli strain for thermoinducible T7Pol-driven transcription has been constructed. The strain was obtained by site-specific integration of the T7 gene 1 coding for T7Po1 into the attB site of phage λ in the E. coli chromosome. The expression of the inserted gene is regulated by cIts857 and major early promoter-operator regions of phage λ.

Escherichia coli strain for thermoinducible T7 RNA polymerase-driven expression