Cytochrome P 450 isoforms involved in melatonin metabolism in human liver microsomes

Objective: The present study was carried out to identify the cytochrome P null null null null null null null null null null null null null null null 450 enzyme(s) involved in the 6-hydroxylation and O-demethylation of melatonin. Methods: The formation kinetics of 6-hydroxymelatonin and N-acetylserotonin were determined using human liver microsomes and cDNA yeast-expressed human enzymes (CYP1A2, 2C9 and 2C19) over the substrate concentration...