MAPK/ERK pathway

Cell biology

Biology

Tuberous sclerosis

Signal transduction

Macrophage polarization

Cancer research

Genetics

TSC1

In vitro

Macrophage

Medicine

PI3K/AKT/mTOR pathway

Pathology

Nature Communications

Macrophages acquire distinct phenotypes during tissue stress and inflammatory responses, but the mechanisms that regulate the macrophage polarization are poorly defined. Here we show that tuberous sclerosis complex 1 (TSC1) is a critical regulator of M1 and M2 phenotypes of macrophages. Mice with myeloid-specific deletion of TSC1 exhibit enhanced M1 response and spontaneously develop M1-related inflammatory disorders. However, TSC1-deficient mice are highly resistant to M2-polarized allergic asthma. Inhibition of the mammalian target of rapamycin (mTOR) fails to reverse the hypersensitive M1 response of TSC1-deficient macrophages, but efficiently rescues the defective M2 polarization. Deletion of mTOR also fails to reverse the enhanced inflammatory response of TSC1-deficient macrophages. Molecular studies indicate that TSC1 inhibits M1 polarization by suppressing the Ras GTPase–Raf1–MEK–ERK pathway in mTOR-independent manner, whereas TSC1 promotes M2 properties by mTOR-dependent CCAAT/enhancer-binding protein-β pathways. Overall, these findings define a key role for TSC1 in orchestrating macrophage polarization via mTOR-dependent and independent pathways. Macrophages can be polarized to inflammatory M1 and anti-inflammatory M2 phenotypes, depending on the cytokine milieu. Here, Zhu et al.demonstrate that tuberous sclerosis complex 1 (TSC1) inhibits M1 and promotes M2 polarization of macrophages to prevent inflammatory disorders.

TSC1 controls macrophage polarization to prevent inflammatory disease