Aglycone

Biology

Sapogenin

Saponin

Protopanaxadiol

Botany

Cellulase

Food science

Trichoderma reesei

Ginseng

Alternative medicine

Dioscorea

Organic chemistry

Sugar

Bioconversion

Hydrolysis

Glycoside

Medicine

Chemistry

Fermentation

Ginsenoside

Pathology

Diosgenin

Advanced materials research

Microbial transformation of plant tubers for diosgenin production is an attractive clean method in industry. The purpose of this study was to evaluate the quality of the product obtained this way. Changes of saponins during the hydrolysis of Dioscorea zingiberensis tubers by Trichoderma reesei were investigated. Four main saponins were isolated from the plant herbs. Sugar chains, which connected to the C-3 hydroxyl group in saponins, were cleaved stepwisely by Trichoderma reesei from the terminal sugar until the aglycone was released. Diosgenin-3-O-β-D-glucose was a byproduct in this process. While, in the petrol extract of the microbial hydrolyzate, only diosgenin was detected. Melting point, IR spectra and 13 C NMR spectroscopy were used to estimate the quality of the product. Diosgenin obtained from bioconversion showed similar characteristic to that from acid hydrolyzation, confirming microbial transformation was an effective method for diosgenin production.

Microbial Transformation of Saponins in &lt;i&gt;Dioscorea zingiberensis&lt;/i&gt; for Diosgenin Production with &lt;i&gt;Trichoderma reesei&lt;/i&gt;

Microbial Transformation of Saponins in &lt;i&gt;Dioscorea zingiberensis&lt;/i&gt; for Diosgenin Production with &lt;i&gt;Trichoderma reesei&lt;/i&gt;

Microbial Transformation of Saponins in <i>Dioscorea zingiberensis</i> for Diosgenin Production with <i>Trichoderma reesei</i>