The specificity of three major polypeptides (35, 33 and 32.5 kD) from Paragonimus heterotremus antigens prepared from ether-extracted adult worms was tested against sera from heterologous infections as well as against P. westermani-infected sera. Only the 35 kD polypeptide was not present, its antigenic determinant being bound to the antibodies from all P. westermani-infected cases. Its cross-reactivity against various sera from heterologous helminthiases and other lung infections showed that it is not bound to these antigenic polypeptides. These major bands cannot be detected by Concanavalin A detector. Our research encourages the pattern (35, 33 and 32.5 kD) of immunoblot reactions for the diagnosis of P. heterotremus infections; the 35 kD antigen is specific for corresponding species and able to differentiate infections between both species of Paragonimus.
Delipidized protein prepared from lyophilized adult worms of Paragonimus heterotremus demonstrated about 40 stained bands with Coomassie brilliant blue after fractionating through 12% SDS-PAGE. Proteins (80 μg/serum sample) were run through SDS-PAGE and EITB with various human antisera from paragonimiasis, other helminthiases, and tuberculosis cases, as well as from normal controls. Results revealed immune complexes with serum IgG of 40 paragonimiasis presenting 9 major molecular weights of appr...
Abstract A 0.6 kb DNA fragment has been isolated from a genomic sublibrary of the cestode Echinococcus multilocularis . This DNA-fragment showed a strong hybridization signal to 32 PdCTP labeled total DNA prepared from E. multilocularis metacestode material. The fragment was subcloned into the Escherichia coli vector Bluescript BS + resulting in the recombinant plasmid pAL1. The recombinant parasite DNA probe was labeled by biotinylation and hybridized to Southern blots of resolved EcoRI/PstI di...
Abstract The subunit composition and specificity of the major Echinococcus granulosus cyst fluid antigens were determined by immunochemical analysis using murine monoclonal antibodies against Antigen 5 and Antigen B and human sera. Immune complexes cut out from immunoelectrophoresis gels and murine hybridomas were used as a source of specific anti-Antigen 5 and anti-Antigen B antibodies. Immunoprecipitation and Western blot analyses in sodium dodecyl sulphate-polyacrylamide gels using these resa...
#1Fadwa M. Al-Yaman(Bernhard Nocht Institute for Tropical Medicine)H-Index: 1
#2Jürgen Knobloch(Bernhard Nocht Institute for Tropical Medicine)H-Index: 22
Last. Jürgen Knobloch(Bernhard Nocht Institute for Tropical Medicine)H-Index: 1
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Abstract Two parasite antigens have been isolated from Echinococcus granulosus hydatid cyst fluid using hydrophobic interaction chromatography, anion exchange chromatography and gel filtration chromatography. Initial characterization of the antigens indicates that both are glycoproteins, of approximately 20 and 48 kDa (Eg20 and Eg48). When the two antigens were tested with a battery of antisera from patients with heterologous parasitic infections, only Eg20 was found to be specific for E. granul...
#2Victor C. W. Tsang(CDC: Centers for Disease Control and Prevention)H-Index: 59
Last. Peter M. Schantz(CDC: Centers for Disease Control and Prevention)H-Index: 64
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Analysis of human serum reactivities to antigenic components of soluble Taenia solium metacestode proteins showed the predominant presence of determinants shared by T. solium, Echinococcus multilocularis and E. granulosus. Two polypeptides were demonstrated by SDS-PAGE and Western blot or enzyme-linked immunoelectrotransfer blot (EITB) assay to bind serum and CSF antibodies only from T. solium cysticercosis patients. These species-specific antigenic polypeptides focused between pH 4.6 and 3.9 af...
Etude faite chez 93 thai et laotiens. 44 hommes et 49 femmes. L'âge variait de 12 a 79 ans. L'histoire de la maladie variant de 4 mois a 14 ans et les œufs expulses par jour variaient de 400 a 300000. 87,1% des sujets presentaient des lesions. 22,2% avait 1 seule lesion et 77,7% des lesions multiples. Les lesions les plus commnunes etaient une formation kystique simple ou multiple et une infiltration lineaire
: A 40-year old Thai farmer with pulmonary paragonimiasis was treated with Praziquantel 25 mg/kg body weight, three times after meals for one day. On follow-up treatment during hospitalization he coughed up 7 adult lung flukes. The worms were identified as Paragonimus heterotremus. This is probably the first case report to demonstrate adult mature Paragonimus coughed up from man.
(1966). The first case of human paragonimiasis caused by Paragonimus heterotremus Chen et Hsia, 1964. Annals of Tropical Medicine & Parasitology: Vol. 60, No. 4, pp. 509-514.
Abstract Paragonimiasis, or lung fluke disease, is a typical food-borne parasitic zoonosis caused by infection with trematodes belonging to the genus Paragonimus. More than 50 species of Paragonimus have been reported throughout the world, of which seven valid species infect humans, an estimated one million people annually worldwide. Among the seven species, P. westermani , P. heterotremus , and P. skrjabini / P. s. miyazakii , distributed in Asia, are the most important species as the cause of ...
Paragonimus heterotremus is the main causative agent of paragonimiasis in Thailand. In Western blot diagnostic assays for paragonimiasis, the 35 kDa band present in crude P. heterotremus somatic extracts represents one of the known diagnostic bands. This study aimed to use a P. heterotremus cDNA library to create a recombinant version of this antigen for use in immunodiagnosis of paragonimiasis. To accomplish this aim a cDNA expression library was constructed from adult worm mRNA and immuno-scre...
: Infection of boar-hunting dogs with Paragonimus westermani was investigated in Western Japan. Blood and rectal feces were collected from 441 dogs in the three districts (205 in Kinki, 131 in Chugoku and 105 in Shikoku District). In a screening ELISA for serum antibody against P. westermani antigen, 195 dogs (44.2%) showed positive reaction. In the 195 dogs, 8 dogs were found excreting P. westermani eggs after molecular analysis of fecal eggs, and additional 7 were identified serologically for ...
Last. M.O. Sato(UFT: Federal University of Tocantins)
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Trematodes are reviewed in groups, based on the source of their infective stage, either vegetables or animals (crustaceans, fish, snails, insects, amphibians, and reptiles). The biology, epidemiology, pathogenesis, and symptoms of infection of species reported in humans are discussed. The chapter concludes with a summary of information on diagnosis, treatment, prevention, and control of these trematodes.
#1Jong-Yil Chai(SNU: Seoul National University)H-Index: 48
Human paragonimiasis is caused by nine species of Paragonimus, namely, P. westermani, P. africanus, P. heterotremus, P. kellicotti, P. mexicanus, P. siamensis, P. skrjabini, P. skrjabini miyazakii, and P. uterobilateralis. Cerebral or spinal involvements are most common in P. westermani, and can also occur in P. skrjabini, P. skrjabini miyazakii, and P. mexicanus. In P. westermani, cerebral paragonimiasis comprises about 45% of all extrapulmonary paragonimiasis cases, and accounts for about 1% o...
A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values...
Paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. In Southeast Asia, Paragonimus heterotremus is the only proven causative pathogen. Recently, a new Paragonimus species, P. pseudoheterotremus, was found in Thailand. This species is genetically similar to P. heterotremus and is considered as a sister species. However, infectivity or pathogenicity of P. pseudoheterotremus to humans remains unclear. We report the first confir...
Ever since the discovery of the first indigenous case in 1981, paragonimiasis has gained recognition as a significant food borne parasitic zoonosis in India. The data available on the occurrence of paragonimiasis, until today, may be just the tip of an iceberg as the study areas covered were restricted to Northeast Indian States. Nevertheless, the results of research on paragonimiasis in India have revealed valuable information in epidemiology, life cycle, pathobiology and speciation of Indian P...
Paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. In Southeast Asia, Paragonimus heterotremus is the only proven causative pathogen. Recently, a new Paragonimus species, P. pseudoheterotremus, was found in Thailand. This species is genetically similar to P. heterotremus and is considered as a sister species. However, infectivity or pathogenicity of P. pseudoheterotremus to humans remains unclear. We report the first confir...
Last. Yukifumi Nawa(KKU: Khon Kaen University)H-Index: 51
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Parasitological and sero-epidemiological surveys for human paragonimiasis were conducted in three provinces of Viet Nam. A total of 590 participants from two known endemic areas of human paragonimiasis (Sinho district of Laichau province and Lucyen district of Yenbai province) and from Dakrong district of Quangtri province where we recently found crab hosts heavily infected with Paragonimus westermani metacercariae. By multiple dot-ELISA screening, 28 (12.7%) out of 220 participants in Sinho dis...