Human lung fluke Paragonimus heterotremus: differential diagnosis between Paragonimus heterotremus and Paragonimus westermani infections by EITB

Published on Jan 1, 1998in Tropical Medicine & International Health2.308
· DOI :10.1046/j.1365-3156.1998.00172.x
Paron Dekumyoy19
Estimated H-index: 19
(MU: Mahidol University),
Jitra Waikagul31
Estimated H-index: 31
(MU: Mahidol University)
+ 0 AuthorsKeeseon S. Eom31
Estimated H-index: 31
(CBNU: Chungbuk National University)
The specificity of three major polypeptides (35, 33 and 32.5 kD) from Paragonimus heterotremus antigens prepared from ether-extracted adult worms was tested against sera from heterologous infections as well as against P. westermani-infected sera. Only the 35 kD polypeptide was not present, its antigenic determinant being bound to the antibodies from all P. westermani-infected cases. Its cross-reactivity against various sera from heterologous helminthiases and other lung infections showed that it is not bound to these antigenic polypeptides. These major bands cannot be detected by Concanavalin A detector. Our research encourages the pattern (35, 33 and 32.5 kD) of immunoblot reactions for the diagnosis of P. heterotremus infections; the 35 kD antigen is specific for corresponding species and able to differentiate infections between both species of Paragonimus.
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