Control of gluconeogenesis in chick (Gallus domesticus) isolated hepatocytes: Effect of redox state and phosphoenolpyruvate carboxykinase (EC 4.1.1.32) location

1. Hepatocytes isolated from 3- to 5-week old chicks synthesized glucose from a variety of precursors. Laetate and fructose proved the most effective with pyruvate less so and glycerol and alanine relatively poor. 2. Oleate inhibited gluconeogenesis from lactate, pyruvate and glycerol. 3. Ethanol inhibited gluconeogenesis from lactate, glycerol, glyceraldehyde and fructose but stimulated gluconeogenesis from pyruvate. All these effects were...