Biology

Cell culture

Caesalpinia

Lipopolysaccharide

Nitric oxide

Anti-inflammatory

Terpenoid

Botany

Stereochemistry

Immunology

Genetics

Organic chemistry

Pharmacology

Dimer

Medicine

Chemistry

Tumor necrosis factor alpha

Two-dimensional nuclear magnetic resonance spectroscopy

Traditional medicine

Phytochemistry

Anti-inflammatory assay-guided separation of extracts from the roots of Caesalpinia mimosoides Lamk. led to isolation of seven compounds: four diterpenes (1–4), a dimer (9), and two dibenzo[b,d]furans (10, 11) together with eleven known compounds. Their structures were elucidated by 1D- and 2D-NMR techniques as well as UV, IR, mass spectral data and comparison with literature values. The anti-inflammatory activities of all compounds were evaluated for inhibitory activities against lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW264.7 cell lines. Compounds 4, 6, 8, and 12–14 were also tested for the inhibitory effect on LPS-induced tumor necrosis factor-alpha (TNF-α) release in RAW264.7 cells. The results indicated that 4 possessed potent inhibitory activity for both tests with IC50 values of 3.0 and 6.5 μM, respectively.

Potential anti-inflammatory diterpenoids from the roots of Caesalpinia mimosoides Lamk.