Association between moderately oxidized low-density lipoprotein and high-density lipoprotein particle subclass distribution in hemodialyzed and post-renal transplant patients.

Published on May 5, 2011in Journal of Zhejiang University-science B2.082
· DOI :10.1631/JZUS.B1000348
Elżbieta Kimak10
Estimated H-index: 10
(Medical University of Lublin),
Magdalena Hałabiś5
Estimated H-index: 5
(Medical University of Lublin)
+ 2 AuthorsAndrzej Książek11
Estimated H-index: 11
(Medical University of Lublin)
Disturbances in the metabolism of lipoprotein profiles and oxidative stress in hemodialyzed (HD) and post-renal transplant (Tx) patients are proatherogenic, but elevated concentrations of plasma high-density lipoprotein (HDL) reduce the risk of cardiovascular disease. We investigated the concentrations of lipid, lipoprotein, HDL particle, oxidized low-density lipoprotein (ox-LDL) and anti-ox-LDL, and paraoxonase-1 (PON-1) activity in HD (n=33) and Tx (n=71) patients who were non-smokers without active inflammatory disease, liver disease, diabetes, or malignancy. HD patients had moderate hypertriglyceridemia, normocholesterolemia, low HDL-C, apolipoprotein A-I (apoA-I) and HDL particle concentrations as well as PON-1 activity, and increased ox-LDL and anti-ox-LDL levels. Tx patients had hypertriglyceridemia, hypercholesterolemia, moderately decreased HDL-C and HDL particle concentrations and PON-1 activity, and moderately increased ox-LDL and anti-ox-LDL levels as compared to the reference, but ox-LDL and anti-ox-LDL levels and PON-1 activity were more disturbed in HD patients. However, in both patient groups, lipid and lipoprotein ratios (total cholesterol (TC)/HDL-C, LDL-C/HDL-C, triglyceride (TG)/HDL-C, HDL-C/non-HDL-C, apoA-I/apoB, HDL-C/apoA-I, TG/HDL) were atherogenic. The Spearman’s rank coefficient test showed that the concentration of ox-LDL correlated positively with HDL particle level (R=0.363, P=0.004), and negatively with TC (R=−0.306, P=0.012), LDL-C (R=−0.283, P=0.020), and non-HDL-C (R=−0.263, P=0.030) levels in Tx patients. Multiple stepwise forward regression analysis in Tx patients demonstrated that ox-LDL concentration, as an independent variable, was associated significantly positively with HDL particle level. The results indicated that ox-LDL and decreased PON-1 activity in Tx patients may give rise to more mildly-oxidized HDLs, which are less stable, easily undergo metabolic remodeling, generate a greater number of smaller pre-β-HDL particles, and thus accelerate reverse cholesterol transport, which may be beneficial for Tx patients. Further studies are necessary to confirm this.
📖 Papers frequently viewed together
11 Citations
12 Citations
97 Citations
#1Nakanishi Shuhei (UH: University of Helsinki)H-Index: 1
#2Sanni Söderlund (UH: University of Helsinki)H-Index: 21
Last. Marja-Riitta Taskinen (UH: University of Helsinki)H-Index: 128
view all 4 authors...
Objectives To study the resistance of HDL particles to direct oxidation in respect to the distribution of HDL particles.
42 CitationsSource
#1E. Samouilidou (Alexandra Hospital)H-Index: 5
#2A. KarpouzaH-Index: 2
Last. H. Tzanatou-ExarchouH-Index: 1
view all 4 authors...
Background: Dyslipoproteinemia and oxidative modification of LDL (oxLDL) are common symptoms in patients suffering from chronic renal failure on hemodialysis (HD), and contribute to
7 CitationsSource
#1Elżbieta Kimak (Medical University of Lublin)H-Index: 10
#2Magdalena Hałabiś (Medical University of Lublin)H-Index: 5
Last. Iwona Baranowicz-Gąszczyk (Medical University of Lublin)H-Index: 4
view all 3 authors...
Objective Disturbances in lipid and lipoprotein profiles in patients after kidney transplantation (Tx) are still not understood.
12 CitationsSource
#1Nagahiko Sakuma (Nagoya City University)H-Index: 14
#2Tomoaki Saeki (Nagoya City University)H-Index: 6
Last. Syuichi Kitada (Nagoya City University)H-Index: 1
view all 8 authors...
Aim: To investigate whether HDL2 can inhibit further oxidative modification of partially oxidized LDL (ox-LDL) by interrupting the chain oxidation reaction after lipid hydroperoxides (LOOH) formation. Methods: Following incubation of LDL 400 μg protein/mL phosphate-buffered saline with Cu2+ for 1.75 h (defined as 0 min), incubation was continued after adding HDL2 200 μg protein/mL or HDL2 800 μg protein/mL to give both ox-LDL+HDL2 200 μg protein/mL or ox-LDL+HDL2 800 μg protein/mL. As a control,...
2 CitationsSource
#1Mungli Prakash (KMC: Kasturba Medical College, Manipal)H-Index: 11
#2Nagaraja M. Phani (KMC: Kasturba Medical College, Manipal)H-Index: 7
Last. M Supriya (KMC: Kasturba Medical College, Manipal)H-Index: 1
view all 4 authors...
Paraoxonase (PON) is an aryldialkylphosphatase, which reversibly binds and hydrolyzes organophosphates. The PON family has three members (PON1, PON2 and PON3); they share structural properties and enzymatic activities. PON1 is shown to reside over high density lipoprotein (HDL) and has both antioxidant and antiatherogenic functions. Function of PON2 and PON3 are speculative and still under research. Several methodologies were developed over the years to determine the activity and mass of PON1, o...
17 CitationsSource
#1Manfredi Rizzo (University of Palermo)H-Index: 64
Last. Jelena VekicH-Index: 15
view all 8 authors...
Although results from in vitro studies and clinical trials demonstrate strong associations between oxidative stress and cardiovascular risk, to date still no convincing data are available to suggest that treatment with antioxidants might reduce vascular events. Oxidative modifications of low-density lipoproteins (LDL) represent an early stage of atherosclerosis, and small, dense LDL are more susceptible to oxidation than larger, more buoyant particles. Oxidized LDL are independent predictors of ...
87 CitationsSource
#1Hamid Moradi (UCI: University of California, Irvine)H-Index: 25
#1Hamid Moradi (UCI: University of California, Irvine)
Last. Nosratola D. Vaziri (UCI: University of California, Irvine)H-Index: 117
view all 4 authors...
Chronic kidney disease (CKD) is associated with accelerated atherosclerosis and increased mortality from cardiovascular disease. CKD results in oxidative stress, inflammation, and high-density lipoprotein (HDL) deficiency, which work in concert to promote atherosclerosis. Normal HDL confers protection against atherosclerosis by inhibiting the oxidation of lipids and lipoproteins and by retrieving surplus cholesterol and phospholipids from lipid-laden cells in the artery wall for disposal in the ...
139 CitationsSource
#1Won Suk An (Dong-a University)H-Index: 12
#2Seong-Eun Kim (Dong-a University)H-Index: 3
Last. Seo-Hee Rha (Dong-a University)H-Index: 10
view all 5 authors...
Cardiovascular mortality is associated with vascular calcification (VC) in hemodialysis (HD) patients. The present study was designed to find factors related with medial artery calcification on the plain radiography of feet by comparing C-reactive protein (CRP), plasminogen activator inhibitor type 1 (PAI-1) and lipid profile including oxidized low density lipoprotein (ox-LDL) and to elucidate associations among these factors in HD patients. Forty-eight HD patients were recruited for this study....
20 CitationsSource
#1Madhumita Guha (BU: Boston University)H-Index: 6
#2Xuan GaoH-Index: 5
Last. Olga GurskyH-Index: 26
view all 4 authors...
High-density lipoproteins (HDLs) are protein−lipid assemblies that remove excess cell cholesterol and prevent atherosclerosis. HDLs are stabilized by kinetic barriers that decelerate protein dissociation and lipoprotein fusion. We propose that similar barriers modulate metabolic remodeling of plasma HDLs; hence, changes in particle composition that destabilize HDLs and accelerate their denaturation may accelerate their metabolic remodeling. To test this notion, we correlate existing reports on H...
31 CitationsSource
Plasma concentration of high density lipoprotein (HDL) is one of the most reliable negative risk factors for CVD. There is however convincing experimental and clinical evidence that plasma concentration of HDL does not convey the full picture of atheroprotective properties of HDL. HDL functionality, i.e. the ability of HDL to perform its many atheroprotective functions, is partly independent of HDL concentration and may be as important, if not more important, in determining the atheroprotective ...
94 CitationsSource
Cited By12
Abstract null null Chronic systemic inflammation is associated with an increased risk of cardiovascular disease in an environment of low low-density lipoprotein (LDL) and low total cholesterol and with the pathophysiology of neuroprogressive disorders. The causes and consequences of this lipid paradox are explored. Circulating activated neutrophils can release inflammatory molecules such as myeloperoxidase and the pro-inflammatory cytokines interleukin-1 beta, interleukin-6 and tumour necrosis f...
#1Elżbieta KimakH-Index: 10
#2Bartosz ZiębaH-Index: 2
Last. Janusz SolskiH-Index: 13
view all 4 authors...
Background Myeloperoxidase (MPO) impairing endothelial functions. We investigated whether increasing concentration of myeloperoxidase (MPO) and inflammatory markers induce progression and incident acute coronary syndrome (ACS) in stable coronary artery disease (SCAD) patients. Therefore, the concentration of MPO, lipids, lipoproteins (apo(apolipoprotein) AI, apoB, lipoprotein associated phospholipase A2 (LpPLA2) level), inflammatory markers (high sensitivity C-reactive protein (hsCRP), tumor nec...
9 CitationsSource
#1Hossein Tabriziani (LLU: Loma Linda University)H-Index: 1
#2Michael S. Lipkowitz (Georgetown University)H-Index: 31
Last. Nhan Vuong (LLUMC: Loma Linda University Medical Center)H-Index: 1
view all 3 authors...
: Oxidative stress plays a key role in the pathophysiological process of uremia and its complications, particularly in cardiovascular disease. The level of oxidative stress markers is known to increase as chronic kidney disease progresses and correlates significantly with the level of renal function. Hemodialysis and peritoneal dialysis are major modes of renal replacement therapy for end-stage renal disease patients, but unfortunately they are also accompanied by increased oxidative stress. Suc...
15 CitationsSource
End stage-renal-disease (ESRD) is associated with dyslipidemia and premature atherosclerosis. The study evaluates the effect of hemodialysis (HD) on HDL-remodeling between pre- and post-HD. Was conducted a cross-sectional study with 50 ESRD male patients, undergoing HD at Ana Neri Hospital, Salvador, Brazil. All individuals were on HD for at least 3 months, into a three sessions protocol for 3 - 4 hours per week, with a polysulfone low-flow basic-dialyzing-membrane and unfractionated-heparin. HD...
#1Elżbieta Kimak (Medical University of Lublin)H-Index: 10
#2Grzegorz Dzida (Medical University of Lublin)H-Index: 12
Last. Agnieszka Kowalska (Medical University of Lublin)H-Index: 1
view all 9 authors...
2 CitationsSource
#1Elżbieta KimakH-Index: 10
#2Jerzy BylinaH-Index: 1
Last. Andrzej KsiążekH-Index: 11
view all 6 authors...
High-density lipoprotein (HDL) remodeling within the plasma compartment and the association between lecithin-cholesterol acyltransferase (LCAT) and cholesterol ester transfer protein (CETP) activity, and lipid, lipoprotein concentrations and composition were investigated. The aim was to examine the high sensitivity of C-reactive protein (hsCRP), lipid, apolipoprotein B (apoB), apoAI, total apoAII, apoAIInonB, apoB-containing apoAII (apoB:AII), total apoCIII, apoCIIInonB, apoB-containing apoCIII ...
7 CitationsSource
Background/Aims: Cardiovascular diseases are the major cause of morbidity and mortality in hemodialysis (HD) patients. These patients present reduced paraoxonase
14 CitationsSource