T-Cell Clonality in Pityriasis Lichenoides et Varioliformis Acuta: A Heteroduplex Analysis of 20 Cases

Published on Dec 1, 2000in Archives of Dermatology
1.47
· DOI :10.1001/archderm.136.12.1483
Olivier Dereure5
Estimated H-index: 5
(Harvard University),
Edi Levi27
Estimated H-index: 27
(),
Marshall E. Kadin67
Estimated H-index: 67
()
Sources
Abstract
nant T-cell clone by polymerase chain reaction and heteroduplex analysis targeted on the TCRγ gene. Peripheral blood mononuclear cells (PBMCs) and Jurkat cells were used as negative and positive controls. Serial dilutions of Jurkat T-cell lymphoma DNA in PBMC DNA were used to assess the sensitivity of the method. Results: Analysis of 13 (65%) of 20 PLEVA biopsy specimens revealed the presence of a dominant T-cell clone. Positive and negative controls confirmed the specificity of the procedure. The sensitivity was determined to be between 1% and 5% of the total T-cell infiltrate. Conclusions: This study provides further evidence for the presence of a dominant T-cell clone in skin lesions of some patients with PLEVA and supports the hypothesis that PLEVA is part of the spectrum of clonal‐T-cell cutaneous lymphoproliferative disorders. Arch Dermatol. 2000;136:1483-1486
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