Protrusive activity, cytoplasmic compartmentalization, and restriction rings in locomoting blebbing Walker carcinosarcoma cells are related to detachment of cortical actin from the plasma membrane

The dynamic events at the front of locomoting blebbing Walker carcinosarcoma cells [Keller and Bebie, Cell Motil. Cytoskeleton 33:241-251, 1996] are interpreted on the basis of an analysis of the actin cytoskeleton and its relationship to the plasma membrane in fixed cells using a novel double-staining procedure. The data show that blebs are formed where cortical actin is locally depolymerized and/or by detachment of the plasma membrane from...